Biofilms with fungi in chronic rhinosinusitis
Biofilms with fungi in chronic rhinosinusitis
复制标题
DOI：
10.1016/j.otohns.2008.02.002
复制
发表时间：
2008-05-01
影响因子：
3.4
通讯作者：
Hunsaker, Darrell H.
中科院分区：
文献类型：
--
作者：
Healy, David Y.;Leid, Jeff G.;Hunsaker, Darrell H.
目的：证明嗜酸性粘蛋白慢性鼻窦炎（EMCRS）和变应性真菌性鼻窦炎（AFRS）患者鼻窦粘膜样本中的细菌生物膜含有真菌成分;鉴定生物膜中的特定生物体。根据组织病理学检查结果，将患者分为AFRS、EMCRS或慢性鼻窦炎（CRS）。还收集了来自对照的三份粘膜样品。用特异性细菌荧光原位杂交（FISH）DNA探针（流感嗜血杆菌、嗜肺链球菌、金黄色葡萄球菌和铜绿假单胞菌）和通用泛真菌FISH探针对样本进行染色。结果：9/11例标本和2/3例对照标本中发现细菌生物被膜。H.流感病毒是存在的主要生物膜。AFRS和EMCRS生物膜中的真菌成分比CRS和对照组多。结论：这是一项初步研究，证实了鼻窦粘膜生物膜中的真菌成分和AFRS中的生物膜。(c)2008年美国耳鼻咽喉头颈外科学会基金会。All rights reserved.
OBJECTIVES: Demonstrate that bacterial biofilm in sinus mucosal samples from patients with eosinophilic mucin chronic rhinosinusitis (EMCRS) and allergic fungal rhinosinusitis (AFRS) contains fungal elements; identify specific organisms in the biofilm.METHODS: Mucosa samples from 11 patients undergoing sinus surgery were collected. Patients were classified as having AFRS, EMCRS, or chronic rhinosinusitis (CRS) based on histopathologic findings. Three mucosal samples from controls were also collected. Samples were stained with specific bacterial fluorescent in situ hybridization (FISH) DNA probes (Haemophilus influenzae, Streptococcus pneumophilia, Staphylococcus aureus, and Pseudomonas aeruginosa) and a general pan-fungal FISH probe. The samples were analyzed for bacterial biofilm ultrastructure and fungal elements using epifluorescent microscopy.RESULTS: Bacterial biofilm was demonstrated in 9/11 samples and 2/3 controls. H. influenzae was the predominant biofilm present. There was a trend showing more fungal elements in AFRS and EMCRS biofilms than in CRS and controls.CONCLUSION: This is a preliminary study demonstrating fungal elements within sinus mucosal biofilm and demonstrating biofilm in AFRS. (c) 2008 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.