Kinetics of hydrogen peroxide elimination by astrocytes and C6 glioma cells - Analysis based on a mathematical model
Kinetics of hydrogen peroxide elimination by astrocytes and C6 glioma cells - Analysis based on a mathematical model
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DOI：
10.1016/j.bbagen.2008.03.010
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发表时间：
2008-06-01
影响因子：
3
通讯作者：
Sagara, Jun-ichi
中科院分区：
文献类型：
--
作者：
Makino, Nobuo;Mise, Takeshi;Sagara, Jun-ichi
氧化应激与包括神经退行性疾病在内的多种疾病有关，而H2 O2在活性氧和氧化应激的产生中是重要的。在这项研究中，我们已经研究了细胞外H2 O2消除率和相关酶活性在培养的星形胶质细胞和C6胶质瘤细胞，并分析了结果的基础上的数学模型。与其他类型的培养细胞相比，星形胶质细胞具有较高的谷胱甘肽过氧化物酶（GPx）活性，但较低的谷胱甘肽回收活性。C6细胞表现出相对较低的GPx活性，和处理的C6细胞与二丁酰-cAMP，诱导星形胶质细胞分化，增加过氧化氢酶活性和H2 O2渗透率，但对其他酶的活性几乎没有影响。数学模型[N]。Makino，K.佐佐木N Hashida，Y. Sakakura，描述哺乳动物细胞消除H2 O2的代谢模型，包括H2 O2透过细胞质和过氧化物酶体膜的渗透：与实验数据的比较，Biochim。Biophys. Acta 1673（2004）149-159.]，包括相关酶和H_2O_2透过膜的过程，以透过速率常数为变量，可以很好地拟合去除反应的H_2O_2浓度依赖性。与作为神经元培养模型的PC 12细胞相比，在生理H2 O2浓度下星形胶质细胞的H2 O2清除活性显著更高。有关数学模型的详情载于附录。(c)2008 Elsevier B. V.保留所有权利。
Oxidative stress is implicated in a variety of disorders including neurodegenerative diseases, and H2O2 is important in the generation Of reactive oxygen and oxidative stress. In this study, we have examined the rate of extracellular H2O2 elimination and relevant enzyme activities in cultured astrocytes and C6 glioma cells and have analyzed the results based on a mathematical model. As compared with other types of cultured cells, astrocytes showed higher activity of glutathione peroxidase (GPx) but lower activities for GSH recycling. C6 cells showed relatively low GPx activity, and treatment of C6 cells with dibutyryl-cAMP, which induces astrocytic differentiation, increased catalase activity and H2O2 permeation rate but exerted little effect on other enzyme activities. A mathematical model [N. Makino, K. Sasaki, N. Hashida, Y. Sakakura, A metabolic model describing the H2O2 elimination by mammalian cells including H2O2 permeation through cytoplasmic and peroxisomal membranes: comparison with experimental data, Biochim. Biophys. Acta 1673 (2004) 149-159.], which includes relevant enzymes and H2O2 permeation through membranes, was found to be fitted well to the H2O2 concentration dependences of removal reaction with the permeation rate constants as Variable parameters. As compared with PC12 cells as a culture model for neuron, H2O2 removal activity of astrocytes was considerably higher at physiological H2O2 concentrations. The details of the mathematical model are presented in Appendix. (c) 2008 Elsevier B.V. All rights reserved.