Perfused rat isolated anterior pituitary cell column as bioassay for factor(s) controlling release of adrenocorticotropin: validation of a technique.
Perfused rat isolated anterior pituitary cell column as bioassay for factor(s) controlling release of adrenocorticotropin: validation of a technique.
复制标题
灌注大鼠分离垂体前叶细胞柱作为控制促肾上腺皮质激素释放的因子的生物测定：技术验证。
DOI：
10.1210/endo-103-2-521
复制
发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
P. Lowry
中科院分区：
文献类型：
--
作者：
G. Gillies;P. Lowry
A suspension of isolated rat anterior pituitary cells, with viability of 90% was mixed with 0.5 g preswollen Bio-Gel P2 and packed into a column. ACTH in the fractions collected from the perfused column was measured by immunoassay and bioassay with well correlated results (r = 0.84,P = 0.01). Cells were stimulated with 2–4-min pulses or crude stalk median eminence extract (SME) and responses were recorded as total ACTH released in excess of background. A log doseresponse curve was obtained over the range 0.0025–0.1 SME/ml (one SME = extract of 3–4 mg tissue/ml), giving a highly significant regression of response on dose (P < 0.01) with an index of precision of 0.164 ± 0.007 (mean ± SEM, n = 8). LH, as measured by immunoassay, was also released in response to SME. No significant ACTH release was seen with a cortex extract. ACTH elease was stimulated by 56 mM potassium ions; SMEstimulated ACTH release was dependent on calcium and magnesium ions and was inhibited by the presence of corticosterone (0.2 μg/ml)...
