Radioimmunoassay (RIA) of Serum Corticosterone in Rat 1 2
Radioimmunoassay (RIA) of Serum Corticosterone in Rat 1 2
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DOI：
10.3181/00379727-170-41391
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发表时间：
1982-05
影响因子：
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通讯作者：
A. Gwosdow-Cohen;C. L. Chen;E. L. Besch
中科院分区：
文献类型：
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作者：
A. Gwosdow-Cohen;C. L. Chen;E. L. Besch
本文报道了用皮质酮抗血清（377，Niswender）和简单的乙醇提取法测定皮质酮的放射免疫分析方法。抗血清似乎是高度特异性的皮质酮。在50 pg皮质酮时，试验内变异性为3.67 ± 0.75%（平均值± SE）;平均值为51.15 pg时，试验间变异性为6.98 ± 1.01%。测定灵敏度为9.48 ± 0.60 pg。利用该测定，从肾上腺切除和肾上腺切除-卵巢切除大鼠中获得的血清产生的皮质酮值低于完整或卵巢切除大鼠的血清。完整的女性有较低的皮质酮值比完整的男性。暴露于高温（32.5 °）的大鼠血浆皮质酮水平（48.48 ± 4.37 μ g/100 ml）显著高于对照（24.5 °）动物（21.31 ± 2.02 μ g/100 ml）（P <0.001）。高特异性，灵敏度，精密度，回收水平，以及这种技术的易用性，使其适用于血清或血浆皮质酮的研究。
Abstract A radioimmunoassay for corticosterone was developed and characterized using corticosterone antiserum (377, Niswender) and a simple ethanol extraction procedure. The antiserum appeared to be highly specific for corticosterone. Intraassay variability was 3.67 ± 0.75% (mean ± SE) at 50 pg of corticosterone; interassay variability with a mean value of 51.15 pg was 6.98 ± 1.01%. Assay sensitivity was 9.48 ± 0.60 pg. Utilizing this assay, serum obtained from adrenalectomized and adrenalectomized-ovariectomized rats yielded lower corticosterone values than serum from intact or ovariectomized rats. Intact females had lower corticosterone values than intact males. Rats exposed to elevated temperature (32.5°) displayed significantly (P < 0.001) elevated plasma corticosterone levels (48.48 ± 4.37 μg/100 ml) compared to control (24.5°) animals (21.31 ± 2.02 μg/100 ml). The high specificity, sensitivity, precision, recovery level, and ease of this technique make it useful for the study of either serum or plasma corticosterone.