MOLECULAR-CLONING AND EXPRESSION OF A RAT VIA ARGININE VASOPRESSIN RECEPTOR
MOLECULAR-CLONING AND EXPRESSION OF A RAT VIA ARGININE VASOPRESSIN RECEPTOR
复制标题
DOI：
10.1038/356523a0
复制
发表时间：
1992-04-09
期刊：
影响因子：
64.8
通讯作者：
LOLAIT, SJ
中科院分区：
文献类型：
--
作者：
MOREL, A;OCARROLL, AM;LOLAIT, SJ
神经垂体激素精氨酸加压素具有多种作用1-7，包括抑制利尿、收缩平滑肌、刺激肝糖分解和调节垂体促肾上腺皮质激素释放。精氨酸加压素受体是G蛋白偶联的，并且已被分为至少三种类型8; V1 a（血管/肝）1-9-11和V1 b（垂体前叶）12受体，其通过磷脂酰肌醇水解作用以动员细胞内Ca 2+，以及V2（肾）受体1、13、14，其与腺苷酸环化酶偶联。我们在这里报告的互补DNA编码肝V1 a精氨酸加压素受体的克隆。肝脏cDNA编码一种具有7个假定跨膜结构域的蛋白质，该蛋白质结合精氨酸加压素和相关化合物，其亲和力与天然大鼠V1 a受体相似。对应于cDNA的信使RNA分布在已知含有V1 a受体的大鼠组织中。
THE neurohypophyseal hormone arginine vasopressin has diverse actions 1-7, including the inhibition of diuresis, contraction of smooth muscle, stimulation of liver glycogenolysis and modulation of adrenocorticotropic hormone release from the pituitary. Arginine vasopressin receptors are G protein-coupled and have been divided into at least three types 8; the V1a (vascular/hepatic) 1-9-11 and V1b (anterior pituitary) 12 receptors which act through phosphatidylinositol hydrolysis to mobilize intracellular Ca2+, and the V2 (kidney) receptor 1,13,14 which is coupled to adenylate cyclase. We report here the cloning of a complementary DNA encoding the hepatic V1a arginine vasopressin receptor. The liver cDNA encodes a protein with seven putative transmembrane domains, which binds arginine vasopressin and related compounds with affinities similar to the native rat V1a receptor. The messenger RNA corresponding to the cDNA is distributed in rat tissues known to contain V1a receptors.