Genetic variability of Rickettsia spp. in Dermacentor and Haemaphysalis ticks from the Russian Far East
Genetic variability of Rickettsia spp. in Dermacentor and Haemaphysalis ticks from the Russian Far East
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DOI：
10.1016/j.ttbdis.2018.07.015
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发表时间：
2018-09-01
影响因子：
3.2
通讯作者：
Tikunova, Nina
中科院分区：
文献类型：
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作者：
Igolkina, Yana;Rar, Vera;Tikunova, Nina
俄罗斯远东地区是蜱传立克次体病的流行地区。然而，该地区立克次体的流行和遗传变异尚未得到广泛的研究。在这项研究中，188 Dermacentor silvarum，439 Haemaphysalis concinna，和374 Haemaphysalis japonica成年蜱收集了来自俄罗斯远东地区的哈巴罗夫斯克省的4个地点和阿穆尔州的3个地点。检查这些蜱是否存在立克次体属。通过扩增gltA基因的片段通过对gltA、16 S rRNA、ompA、ompB和sca 4基因进行测序，对鉴定的立克次体进行基因分型。黑龙江立克次体（Rickettsiaheilongjiangensis）是远东地区蜱传立克次体病的病原体，检出率为10.5%。concinna和H.日本蜱，而西伯利亚斑疹伤寒的病原体西伯利亚立克次体仅在一株H. concinna滴答声。此外，在D.森林蜱（> 70%）和血蜱（< 3%）中的频率明显较低。在所有检查的蜱种中均发现“塔氏立克次体”（1.6-5.3%）。值得注意的是，这项研究是第一次观察到的“datus R。tarasevichiae”在D.森林蜱。此外，首次在H.在日本蜱中首次发现了埃氏立克次体DNA; concinna和H.日本蜱在血蜱中发现了“主要立克次体”和“稀有立克次体”。滴答声。“Aidatus R. principis”与H. japonica，检出率为5.6%; rara”在H. concinna（3.0%）与H.日本蜱（1.1%）。在本研究中，“datus R. principis”和“R. rara”的16 S rRNA、ompA、ompB和sca 4基因首次被鉴定。
The Russian Far East is an endemic region for tick-borne rickettsioses. However, the prevalence and genetic variability of Rickettsia species in this region have not been extensively investigated. In this study, 188 Dermacentor silvarum, 439 Haemaphysalis concinna, and 374 Haemaphysalis japonica adult ticks were collected from four locations in Khabarovsk Province and three locations in Amur Province in the Russian Far East. These ticks were examined for the presence of Rickettsia spp. by amplifying a fragment of the gltA gene. Identified rickettsiae were genotyped by sequencing of the gltA, 16S rRNA, ompA, ompB, and sca4 genes. In the examined ticks, Rickettsia heilongjiangensis, the causative agent of Far-Eastern tick-borne rickettsiosis, was found in 10.5% of H. concinna and in 1.9% of H. japonica ticks, while Rickettsia sibirica, the agent of Siberian tick typhus, was detected in only one H. concinna tick. In addition, Rickettsia raoultii was found predominantly in D. silvarum ( > 70%) and significantly less frequently in Haemaphysalis ticks ( < 3%). "Candidatus Rickettsia tarasevichiae" was found in all examined tick species (1.6-5.3% in different species). Notably, this study is the first observation of "Candidatus R. tarasevichiae" in D. silvarum ticks. Moreover, DNA of Rickettsia canadensis was found for the first time in a H. japonica tick; DNA of Rickettsia aeschlimannii was revealed for the first time in H. concinna and H. japonica ticks. "Candidatus Rickettsia principis" and "Candidatus Rickettsia rara" were found in Haemaphysalis spp. ticks. "Candidatus R. principis" was associated with H. japonica and identified in 5.6% of the examined ticks, while "Candidatus R. rara" was found more frequently in H. concinna (3.0%) compared to H. japonica ticks (1.1%). In this study, "Candidatus R. principis" and "Candidatus R. rara" were characterized for the first time by the 16S rRNA, ompA, ompB, and sca4 genes.