Fission yeast Adf1 is necessary for reassembly of actin filaments into the contractile ring during cytokinesis.
Fission yeast Adf1 is necessary for reassembly of actin filaments into the contractile ring during cytokinesis.
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DOI：
10.1016/j.bbrc.2018.07.156
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发表时间：
2018-11
影响因子：
3.1
通讯作者：
Eiichi Ueda;J. Kashiwazaki;Saki Inoué;I. Mabuchi
中科院分区：
文献类型：
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作者：
Eiichi Ueda;J. Kashiwazaki;Saki Inoué;I. Mabuchi
ADF/cofilin家族蛋白在体外快速分解肌动蛋白，并且被认为参与细胞中的各种肌动蛋白动力学。Adf 1是在裂殖酵母中表达的该家族蛋白质的成员，并且被认为在肌动蛋白斑块动力学和胞质分裂期间的收缩环形成中发挥作用。我们的目的是利用温度敏感突变体adf 1 - 1来详细了解该蛋白在胞质分裂中的功能。Adf 1在限制性温度下失活后，在G2期晚期，细胞末端出现一簇肌动蛋白斑块。很明显，灭活仅在几分钟内发生。此外，我们发现肌动蛋白簇迁移到分裂后期的网站可能是由两个肌球蛋白5-1和肌球蛋白II的功能。然而，迁移的肌动蛋白簇没有组织成收缩环。当Adf 1在收缩环组装前的中后期B失活时，环不形成，但当Adf 1在该点之后失活时，环形成。我们的结论是，Adf 1的功能在间期肌动蛋白的动力学和有丝分裂过程中收缩环的形成。
ADF/cofilin family proteins quickly disassemble actinin vitro, and are thought to be involved in various actin dynamics in the cell. Adf1 is a member of this family proteins expressed in fission yeast, and is thought to play roles in actin patch dynamics and also contractile ring formation during cytokinesis. We aimed to understand the function of this protein in cytokinesis in detail using the temperature-sensitive mutantadf1-1.Adf1 inactivation at a restrictive temperature during late G2 phase led to a clustering of actin patches at the cell ends. It was apparent that the inactivation occurred only in a few minutes. Furthermore, we found that the actin clusters migrated to the division site during anaphase possibly by the function of both myosin 5-1 and a myosin II. The migrated actin clusters, however, were not organized into the contractile ring. When Adf1 was inactivated at mid-anaphase B before contractile ring assembly, the ring was not formed, but it was formed when Adf1 was inactivated after this point. We conclude that Adf1 functions in the interphase actin dynamics and formation of the contractile ring during mitosis.