A comparison of detection methods for infectious haematopoietic necrosis virus.
A comparison of detection methods for infectious haematopoietic necrosis virus.
复制标题
传染性造血坏死病毒检测方法比较
DOI:
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发表时间:
1985
期刊:
影响因子:
--
通讯作者:
J. Leong
中科院分区:
文献类型:
--
作者:
Y. Hsu;J. Leong
. A comparative study of immunological methods for detecting infectious haematopoietic necrosis virus (IHNV) was made. The anti–IHNV antibody titre was measured by solid phase direct binding assays with‘125iodinated Protein A from Staphylococcus aureus and with immunoperoxidase staining. The binding antibody titre was much higher than that obtained in the virus neutralization assay. The high binding antibody titre of rabbit anti–IHNV sera made the development of two immunological tests for IHNV possible. Virus–specific proteins were detected on nitrocellulose membranes after transfer from denaturing polyacrylamide gels. The immunological methods were highly specific, sensitive lo less than 10 ng of virus protein, and were useful in characterizing the different strains of IHNV.
DOI:
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发表时间:
1981
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
Bollum,FJ;Chang,LM
通讯作者:
Chang,LM
DOI:
10.1073/pnas.78.1.177
发表时间:
1981
影响因子:
11.1
作者:
Symington,J;Green,M;Brackmann,K
通讯作者:
Brackmann,K