A comparison of detection methods for infectious haematopoietic necrosis virus.

A comparison of detection methods for infectious haematopoietic necrosis virus.
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传染性造血坏死病毒检测方法比较

DOI:
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发表时间:
1985
期刊:
影响因子:
--
通讯作者:
J. Leong
J. Leong
中科院分区:
--
文献类型:
--
作者:
Y. Hsu;J. Leong

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。对感染性造血坏死病毒(IHNV)的免疫学检测方法进行了比较研究。采用固相直接结合法测定抗ihnv抗体滴度,并采用免疫过氧化物酶染色法检测金黄色葡萄球菌“125碘化蛋白A”。结合抗体滴度远高于病毒中和试验。兔抗IHNV血清的高结合抗体滴度使两种IHNV免疫检测方法的开发成为可能。从变性聚丙烯酰胺凝胶转移后,在硝化纤维素膜上检测到病毒特异性蛋白。该方法特异性强,对病毒蛋白的敏感性低于10 ng,可用于不同病毒株的鉴定。
. A comparative study of immunological methods for detecting infectious haematopoietic necrosis virus (IHNV) was made. The anti–IHNV antibody titre was measured by solid phase direct binding assays with‘125iodinated Protein A from Staphylococcus aureus and with immunoperoxidase staining. The binding antibody titre was much higher than that obtained in the virus neutralization assay. The high binding antibody titre of rabbit anti–IHNV sera made the development of two immunological tests for IHNV possible. Virus–specific proteins were detected on nitrocellulose membranes after transfer from denaturing polyacrylamide gels. The immunological methods were highly specific, sensitive lo less than 10 ng of virus protein, and were useful in characterizing the different strains of IHNV.
末端脱氧核苷酸转移酶保守结构的免疫学检测。
DOI: --
发表时间: 1981
期刊: The Journal of biological chemistry
影响因子: --
作者:
Bollum,FJ;Chang,LM
通讯作者: Chang,LM
从凝胶电泳转移到重氮纸后蛋白质的免疫放射自显影检测:腺病毒编码蛋白质的分析。
DOI: 10.1073/pnas.78.1.177
发表时间: 1981
影响因子: 11.1
作者:
Symington,J;Green,M;Brackmann,K
通讯作者: Brackmann,K