Antarctic marine molluscs do have an HSP70 heat shock response
Antarctic marine molluscs do have an HSP70 heat shock response
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DOI：
10.1007/s12192-008-0014-8
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发表时间：
2008-03-01
影响因子：
3.8
通讯作者：
Peck, Lloyd S.
中科院分区：
文献类型：
--
作者：
Clark, Melody S.;Fraser, Keiron P. P.;Peck, Lloyd S.
任何生物体的成功不仅取决于生态位适应，还取决于从动态平衡中生存下来的环境扰动的能力，这种情况通常被描述为压力。虽然对抗“压力”的机制已被描述，但所有类群都普遍描述了热休克蛋白(HSPs)的产生，如HSP70。利用简并聚合酶链式反应(PCR)分别从两种进化分化的南极海洋软体动物(Laternula Elltica和Nacella lininna)、一种双壳类和一种腹足类动物中克隆了HSP70基因家族的成员，该家族包括构成成员(HSC70)和诱导成员(HSP70)以及相关的HSP70家族成员GRP78(葡萄糖调节蛋白，78 kDa)。用定量聚合酶链式反应检测急性热休克实验后HSP70家族成员的表达。这两个物种都表现出显著的HSP70基因表达上调，以响应温度升高。然而，诱导这些反应的温度水平因物种而异(与其自然环境温度相比，椭圆藻为+6-8℃，连环藻为+8-10℃)。椭圆乳杆菌也显示了正在研究的基因的组织特异性表达。以前对南极鱼类的研究表明，它们缺乏经典的热休克反应，诱导形式的HSP70永久表达，在更高的温度条件下不会进一步诱导表达。这项研究表明，其他南极动物的情况并非如此，这两种软体动物表现出可诱导的热休克反应，可能是在它们过去温和的进化过程中设定的水平。
The success of any organism depends not only on niche adaptation but also the ability to survive environmental perturbation from homeostasis, a situation generically described as stress. Although species-specific mechanisms to combat "stress" have been described, the production of heat shock proteins (HSPs), such as HSP70, is universally described across all taxa. Members of the HSP70 gene family comprising the constitutive (HSC70) and inducible (HSP70) members, plus GRP78 (glucose-regulated protein, 78 kDa), a related HSP70 family member, were cloned using degenerate polymerase chain reaction (PCR) from two evolutionary divergent Antarctic marine molluscs (Laternula elliptica and Nacella concinna), a bivalve and a gastropod, respectively. The expression of the HSP70 family members was surveyed via quantitative PCR after an acute 2-h heat shock experiment. Both species demonstrated significant up-regulation of HSP70 gene expression in response to increased temperatures. However, the temperature level at which these responses were induced varied with the species (+6-8C for L. elliptica and +8-10C for N. concinna) compared to their natural environmental temperature). L. elliptica also showed tissue-specific expression of the genes under study. Previous work on Antarctic fish has shown that they lack the classical heat shock response, with the inducible form of HSP70 being permanently expressed with an expression not further induced under higher temperature regimes. This study shows that this is not the case for other Antarctic animals, with the two molluscs showing an inducible heat shock response, at a level probably set during their temperate evolutionary past.