A Molecularly Complete Planar Bacterial Outer Membrane Platform.
A Molecularly Complete Planar Bacterial Outer Membrane Platform.
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DOI：
10.1038/srep32715
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发表时间：
2016-09-07
影响因子：
4.6
通讯作者：
Daniel S
中科院分区：
文献类型：
--
作者：
Hsia CY;Chen L;Singh RR;DeLisa MP;Daniel S
The bacterial outer membrane (OM) is a barrier containing membrane proteins and liposaccharides that fulfill crucial functions for Gram-negative bacteria. With the advent of drug-resistant bacteria, it is necessary to understand the functional role of this membrane and its constituents to enable novel drug designs. Here we report a simple method to form an OM-like supported bilayer (OM-SB), which incorporates native lipids and membrane proteins of gram-negative bacteria from outer membrane vesicles (OMVs). We characterize the formation of OM-SBs using quartz crystal microbalance with dissipation (QCM-D) and fluorescence microscopy. We show that the orientation of proteins in the OM-SB matches the native bacterial membrane, preserving the characteristic asymmetry of these membranes. As a demonstration of the utility of the OM-SB platform, we quantitatively measure antibiotic interactions between OM-SBs and polymyxin B, a cationic peptide used to treat Gram-negative infections. This data enriches understanding of the antibacterial mechanism of polymyxin B, including disruption kinetics and changes in membrane mechanical properties. Combining OM-SBs with microfluidics will enable higher throughput screening of antibiotics. With a broader view, we envision that a molecularly complete membrane-scaffold could be useful for cell-free applications employing engineered membrane proteins in bacterial membranes for myriad technological purposes.
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影响因子：
2.9
作者：
Domingues, Marco M.;Inacio, Rita G.;Santos, Nuno C.
通讯作者：
Santos, Nuno C.
影响因子：
2.9
作者：
KAMIO, Y;NIKAIDO, H
通讯作者：
NIKAIDO, H
影响因子：
3.9
作者：
Costello, Deirdre A.;Hsia, Chih-Yun;Daniel, Susan
通讯作者：
Daniel, Susan
影响因子：
5.6
作者：
Kim, Jae-Young;Doody, Anne M.;DeLisa, Matthew P.
通讯作者：
DeLisa, Matthew P.
影响因子：
3.3
作者：
Clausell, Adria;Garcia-Subirats, Maria;Cajal, Yolanda
通讯作者：
Cajal, Yolanda