Morphological and molecular analyses of the tomato floral mutant leafy inflorescence, a new allele of falsiflora
Morphological and molecular analyses of the tomato floral mutant leafy inflorescence, a new allele of falsiflora
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DOI：
10.1016/j.plantsci.2005.03.007
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发表时间：
2005-07-01
期刊：
影响因子：
5.2
通讯作者：
Kanayama, Y
中科院分区：
文献类型：
--
作者：
Kato, K;Ohta, K;Kanayama, Y
本研究的目的是通过对番茄花突变体叶状花序（lfi）的形态学和分子生物学分析，加深对番茄花发育不同于拟南芥花发育的分子机制的理解。在lfi花序中。茎尖状分生组织具有不确定的生长并发育成无法开花的大叶状花序。在 FALSIFLORA (FA) 开放阅读框的 3' 区域发现了 12 bp 缺失，FALSIFLORA 是 lfi 突变体中拟南芥花分生组织识别基因 LEAFY 的番茄直系同源物。等位性测试表明 lfi 与 falsiflora 等位。因为 FA 基因座控制花分生组织的特性。对几个花器官特征基因进行表达分析，以推断这些基因与 FA 之间的关系。番茄花器官识别基因 TDR6 和 TAG1 的表达在 lfi 花序中受到抑制，表明 FA 诱导番茄花器官识别基因，就像 LEAFY 在拟南芥中所做的那样。此外，FA 促进了 SEP3 直向同源物 TDR5 的诱导，尽管 TDR4 不受 FA 调节。对异位心皮形成（lfi 的独特特征）的研究表明，TAG1（C 组基因）和 TDR5 在心皮形成中发挥重要作用，如在拟南芥中。然而，与拟南芥不同，TDR6（被认为是 B 组基因）的表达也与异位心皮形成相关。 (c) 2005 Elsevier Ireland Ltd. 保留所有权利。
The purpose of this study was to advance understanding of the molecular mechanisms of tomato floral development that differ from those of Arabidopsis, through morphological and molecular biological analyses of the tomato floral mutant leafy inflorescence (lfi). In lfi inflorescences. shoot apex-like meristems have indeterminate growth and develop into large leafy inflorescence that fail to flower. A 12 bp deletion was found in the 3' region of the open reading frame of FALSIFLORA (FA), the tomato ortholog of the Arabidopsis floral meristem identity gene LEAFY in the lfi mutant. An allelism test indicated that lfi is allelic to falsiflora. Because the FA locus controls floral meristem identity. expression analyses of several floral organ identity genes were carried out to deduce the relationship between these genes and FA. Expression of the tomato floral organ identity genes TDR6 and TAG1 was inhibited in lfi inflorescences, suggesting that FA induces tomato floral organ identity genes, as LEAFY does in Arabidopsis. In addition, FA promoted the induction of the SEP3 ortholog, TDR5, although TDR4 was not regulated by FA. The investigation of ectopic carpel formation, a unique feature of lfi, suggested that TAG1 (a group C gene) and TDR5 have important roles in carpel formation, as in Arabidopsis. However, the expression of TDR6, which is thought to be a group B gene, is also associated with ectopic carpel formation, unlike in Arabidopsis. (c) 2005 Elsevier Ireland Ltd. All rights reserved.