ENZYME IMMUNO ASSAY OF THE ESTROGEN-RECEPTOR IN BREAST-CANCER BIOPSY SAMPLES - A COMPARISON WITH ISOELECTRIC-FOCUSING
ENZYME IMMUNO ASSAY OF THE ESTROGEN-RECEPTOR IN BREAST-CANCER BIOPSY SAMPLES - A COMPARISON WITH ISOELECTRIC-FOCUSING
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DOI：
10.3109/02841868609136398
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发表时间：
1986-05-01
期刊：
影响因子：
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通讯作者：
SELLBERG, G
中科院分区：
文献类型：
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作者：
FERNO, M;BORG, A;SELLBERG, G
采用等电聚焦(IF)和酶免疫法(EIA)对127例乳腺癌标本进行雌激素受体(ER)检测。将两种技术进行定量比较，相关系数很高(r_s=0.98；p<；0.001)。然而，当使用EIA而不是IF时，ER回收率平均增加了2.5倍。这一增加似乎受到内质网浓度水平的影响，浓度越高，增加的幅度越小。IF和EIA之间的差异似乎并不是由于绝经状态。EIA的一个可能的应用领域可能是测量服用他莫昔芬的患者样本中的ER。在九个这样的样本中，有五个用EIA发现的可测量ER量比IF高得多。环评也被发现是一种比间接免疫分析更简单、耗时更少的方法。然而，这些优势应该与目前较高的成本进行权衡。
The estrogen receptor (ER) was measured by isoelectric focusing (IF) and enzyme immuno assay (EIA) in 127 breast cancer samples. When comparing the two techniques quantitatively, a very high correlation coefficient was obtained (rs = 0.98; p < 0.001). ER recovery increased, however, by a mean factor of 2.5 when EIA was used instead of IF. This increase seemed to be influenced by the ER concentration level, being lower at higher levels. The difference between IF and EIA did not seem to be due to menopausal status. A possible field of application for EIA might be measurement of ER in samples from patients on tamoxifen therapy. In five out of nine such samples, a considerably higher measurable amount of ER was found with EIA than with IF. EIA was also found to be a simpler and less time-consuming method than IF. These advantages should, however, be weighed against the present higher costs.