Changes in responses of neurons in spinal and medullary subnucleus reticularis dorsalis to acupoint stimulation in rats with visceral hyperalgesia.
Changes in responses of neurons in spinal and medullary subnucleus reticularis dorsalis to acupoint stimulation in rats with visceral hyperalgesia.
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内脏痛觉过敏大鼠脊髓及髓质背网状亚核神经元对穴位刺激反应的变化
DOI：
10.1155/2014/768634
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Huang GF
中科院分区：
文献类型：
--
作者：
Yu LL;Li L;Rong PJ;Zhu B;Qin QG;Ben H;Huang GF
本研究旨在从脊髓和延髓水平探讨穴位致敏现象的机制。实验在成年雄性Sprague-Dawley大鼠上进行，并通过结肠直肠扩张（CRD）产生内脏伤害性刺激。记录宽动态范围（WDR）和网状背亚核（SRD）神经元的活动。本实验观察了电针“足三里-上巨虚”穴前后大鼠脊髓背角（WDR）和背角（SRD）神经元对电针反应的变化。结果表明，内脏伤害性感受可使神经元对穴位刺激的反应更易化。在脊髓背角，电针诱导的WDR神经元的激活进一步增加至106.84 ± 17.33%（1.5mA）（P < 0.001）和42.27 ± 13.10%（6 mA）（P < 0.01）。在延髓，电针诱导的SRD神经元的激活进一步增加，分别为CRD前的63.28 ± 15.96%（1.5mA）（P < 0.001）和25.02 ± 7.47%（6 mA）（P < 0.01）。以上结果提示，WDR和SRD神经元的内脏-躯体会聚促进作用可能是穴位致敏的机制之一。但内脏伤害感受对WDR神经元的增敏作用强于SRD神经元。
The purpose of this study was to explore the mechanism of acupoints sensitization phenomenon at the spinal and medulla levels. Experiments were performed on adult male Sprague-Dawley rats and visceral noxious stimuli was generated by colorectal distension (CRD). The activities of wide dynamic range (WDR) and subnucleus reticularis dorsalis (SRD) neurons were recorded. The changes of the reactions of WDR and SRD neurons to electroacupuncture (EA) on acupoints of “Zusanli-Shangjuxu” before and after CRD stimulation were observed. The results showed that visceral nociception could facilitate the response of neurons to acupoints stimulation. In spinal dorsal horn, EA-induced activation of WDR neurons further increased to 106.84 ± 17.33% (1.5 mA) (P < 0.001) and 42.27 ± 13.10% (6 mA) (P < 0.01) compared to the neuronal responses before CRD. In medulla oblongata, EA-induced activation of SRD neurons further increased to 63.28 ± 15.96% (1.5 mA) (P < 0.001) and 25.02 ± 7.47% (6 mA) (P < 0.01) compared to that before CRD. Taken together, these data suggest that the viscerosomatic convergence-facilitation effect of WDR and SRD neurons may underlie the mechanism of acupoints sensitization. But the sensitizing effect of visceral nociception on WDR neurons is stronger than that on SRD neurons.
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DOI：
10.1155/2012/130802
发表时间：
2012
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Ben H;Li L;Rong PJ;Jin ZG;Zhang JL;Li YH;Li X
通讯作者：
Li X
影响因子：
4
作者：
Verne, G. Nicholas;Price, Donald D.;Callam, Christopher S.;Zhang, Buyi;Peck, Josh;Zhou, QiQi
通讯作者：
Zhou, QiQi
影响因子：
2
作者：
FitzGerald, MP;Koch, D;Senka, J
通讯作者：
Senka, J
影响因子：
2.5
作者：
BERNARD, JF;VILLANUEVA, L;LEBARS, D
通讯作者：
LEBARS, D
影响因子：
9.8
作者：
Kurucsai, Gabor;Joo, Ildiko;Madacsy, Laszlo
通讯作者：
Madacsy, Laszlo