ANTIGENIC AND SEQUENCE-ANALYSIS OF H3 INFLUENZA-VIRUS HEMAGGLUTININS FROM PIGS IN ITALY
ANTIGENIC AND SEQUENCE-ANALYSIS OF H3 INFLUENZA-VIRUS HEMAGGLUTININS FROM PIGS IN ITALY
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DOI：
10.1099/0022-1317-75-2-371
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发表时间：
1994-02-01
影响因子：
3.8
通讯作者：
DONATELLI, I
中科院分区：
文献类型：
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作者：
CASTRUCCI, MR;CAMPITELLI, L;DONATELLI, I
为探讨人甲型H3N2型流感病毒在猪体内维持的可能机制，对7株猪源分离株的血凝素(HAS)进行了核苷酸和氨基酸序列分析，以及HA分子对鸡抗血清和单抗的反应性研究。该猪在抗原性和分子特征方面与A/Victoria/3/75人变种的HA非常相似。这些发现与猪HA基因是从早期的人类H3病毒传播给猪的假设一致，在猪身上，它们在5年的时间里以有限的突变存活下来。将测序数据与猪H3基因序列进行比较，研究不同地理区域分离的猪H3基因之间的遗传关系。根据中国和意大利猪分离病毒的核苷酸序列构建的进化树表明，根据分离的国家不同，猪流感A(H3N2)病毒的HA基因具有不同的起源，如人和禽类，并在不同的谱系中独立进化。这项研究直接支持了这样一种假设，即猪可能是人类流感病毒大流行毒株产生的“混合容器”。
To investigate the possible mechanism of maintenance of old human influenza A (H3N2) viruses in pigs, the haemagglutinins (HAs) of seven isolates from swine were studied by analysis of nucleotide and deduced primary amino acid sequences, as well as reactivity of the HA molecule to chicken antisera and monoclonal antibodies. The swine HAs were closely similar to the HA of the A/Victoria/3/75 human variant as regards antigenic and molecular characteristics. These findings are consistent with the hypothesis that the swine HA genes were transmitted from an early human H3 virus to pigs, where they survived with limited mutations over a period of 5 years. The sequence data were also compared with swine H3 sequences to investigate genetic relationships between the H3 genes from swine viruses isolated in different geographical areas. An evolutionary tree, constructed from the nucleotide sequences of viruses isolated from pigs in China and in Italy, illustrated that, depending on the country of their isolation, the HA genes of swine influenza A (H3N2) viruses have different origins, e.g. human and avian, and evolved independently in different lineages. The study provides direct support for the hypothesis that pigs might serve as a 'mixing vessel' for the generation of pandemic strains of human influenza viruses.