Guinea pig skin, a model for epidermal cellular and molecular changes induced by UVR in vivo and in vitro: effects on Mycobacterium bovis Bacillus Calmette-Guerin vaccination.
Guinea pig skin, a model for epidermal cellular and molecular changes induced by UVR in vivo and in vitro: effects on Mycobacterium bovis Bacillus Calmette-Guerin vaccination.
复制标题
豚鼠皮肤，体内外 UVR 诱导的表皮细胞和分子变化模型：对牛分枝杆菌卡介苗疫苗接种的影响。
DOI：
10.1111/j.1751-1097.2012.01218.x
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发表时间：
2013
影响因子：
3.3
通讯作者：
Dirisala,VijayaR
中科院分区：
文献类型：
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作者：
Jeevan,Amminikutty;Formichella,CathrynR;Russell,KarenE;Dirisala,VijayaR
此前，我们报道了紫外线B辐射（UVR）抑制卡介苗诱导的豚鼠结核分枝杆菌（GP）耐药性。在此，我们研究了紫外线照射（200j m−2）的GP表皮细胞（UV - sup）上清液对GP表皮细胞的细胞和分子变化及其体内效应。bovisBCG接种疫苗。UVR增加了皮肤中有核角质形成细胞的数量，但导致CD25+T细胞比例下降。在脾脏中，UVR导致T细胞亚群的比例下降，包括CD25+T细胞，以及主要组织相容性复合体（MHC） II+和CD14+细胞。同样，还观察到包括IL - 10在内的几种细胞因子mrna的显著上调。此外，UV - sup显著降低了腹膜细胞中MHC II类的表达，并减少了T细胞向ConA的增殖。通过抗IL - 10抗体，PPD的增殖恢复到正常水平。将UV - sup注射到接种过BCG的GP中，可显著降低皮肤试验反应和T细胞对PPD的增殖，上调淋巴结或脾脏中IL - 10、IL - 4、IL - 1β和Foxp3 mrna的表达。因此，全身UVR诱导了深刻的细胞和分子变化，从表皮细胞注射UV - sup可以模拟全身UVR对接种过BCG的GP的影响。
Previously, we reported that ultraviolet B‐radiation (UVR) suppressed Bacillus Calmette–Guérin (BCG) vaccine‐induced resistance toMycobacterium tuberculosisin guinea pigs (GP). Herein, we investigated the cellular and molecular changes within the irradiated GP epidermis and thein vivoeffect of supernatants from UV‐irradiated (200 J m−2) epidermal cells (UV‐sup) onM. bovisBCG vaccination. UVR increased the number of nucleated keratinocytes in the skin, but caused a decrease in the proportions of CD25+T cells. In the spleen, UVR resulted in a decrease in the proportions of T‐cell subsets including CD25+T cells, and major histocompatibility complex (MHC) class II+and CD14+cells. Similarly, significant up‐regulation of several cytokine mRNAs including IL‐10 was also observed. Furthermore, UV‐sup significantly reduced the MHC class II expression in peritoneal cells and reduced T‐cell proliferation to ConA. The proliferation to purified protein derivative (PPD) was restored to normal levels by anti‐IL‐10 antibody. The UV‐sup when injected into BCG‐vaccinated GP significantly diminished the skin test response and T‐cell proliferation to PPD and up‐regulated the expression of IL‐10, IL‐4, IL‐1β and Foxp3 mRNAs in the lymph node or spleen. Thus, whole body UVR induces profound cellular and molecular changes and injection of UV‐sup from epidermal cells mimics the effect of whole body UVR in BCG‐vaccinated GP.