A promoter with an internal regulatory domain is part of the origin of replication in BPV-1.
A promoter with an internal regulatory domain is part of the origin of replication in BPV-1.
复制标题
具有内部调节域的启动子是 BPV-1 复制起点的一部分。
DOI：
10.1126/science.3037693
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Botchan,MR
中科院分区：
文献类型：
--
作者：
Stenlund,A;Bream,GL;Botchan,MR
染色体外元件通过细胞倍增而稳定地维持在恒定的拷贝数，是研究高等真核生物DNA复制调控的良好模式系统。以前的研究已经确定了稳定转化细胞中牛乳头瘤病毒的质粒复制所需的顺式和反式功能。这里，已知为质粒复制起点的顺式显性元件的序列显示含有转录启动子。体外和体内试验都已被用于定义该启动子，并表明位于转录起始位点3 ′端的序列是活性所必需的。这个DNA序列元件，它已被定义为通过删除，符合细胞因子的结合位点，也是复制的功能起点所需的。转录因子如何可能发挥作用的调节DNA复制的可能模式进行了讨论。
Extrachromosomal elements that are stably maintained at a constant copy number through cell doublings are a good model system for the study of the regulation of DNA replication in higher eukaryotes. Previous studies have defined both cis and trans functions required for the regulated plasmid replication of the bovine papilloma virus in stably transformed cells. Here, a sequence known to be a cis-dominant element of the replication origin of the plasmid is shown to contain a promoter for transcription. Both in vitro and in vivo assays have been used to define this promoter and show that a sequence located just 3′ to the transcriptional start site is required for activity. This DNA sequence element, which has been defined through deletions, coincides with a binding site for a cellular factor and is also required for a functional origin of replication. Possible models for how a transcription factor may play a role in the regulation of DNA replication are discussed.