Ectopic expression of LBD15 affects lateral branch development and secondary cell wall synthesis in Arabidopsis thaliana
Ectopic expression of LBD15 affects lateral branch development and secondary cell wall synthesis in Arabidopsis thaliana
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DOI：
10.1007/s10725-013-9873-9
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发表时间：
2014-06
影响因子：
4.2
通讯作者：
Lin Zhu;Jiansheng Guo;Cheng Zhou;Jian Zhu
中科院分区：
文献类型：
--
作者：
Lin Zhu;Jiansheng Guo;Cheng Zhou;Jian Zhu
侧器官边界域 (LBD)/ASL（AS2 样）基因编码植物特异性转录因子，在植物生长和发育中发挥重要作用。然而，关于拟南芥LBD15基因的信息很少。在此，评估了LBD15基因的表达模式并研究了其在拟南芥中的生理作用。 LBD15基因在许多组织中以不同水平表达，包括芽、茎、根、花和长角果。 LBD15的过表达可以激活BOP1和BOP2的转录，这对于控制侧器官发育和花序结构至关重要。此外，我们的结果表明，次生壁合成的关键调节因子SND1可以与LBD15启动子的特定SNBE元件结合以激活LBD15的表达。发现LBD15的表达升高会下调CesA4、CesA7和CesA8的转录，这些基因编码次生细胞壁的纤维素合成，并破坏次生细胞壁的形成。由此可见，LBD15可能参与调节木质部细胞分化过程中的次生细胞壁合成。本研究提供了新的证据，可能有助于理解拟南芥侧枝发育和次生细胞壁合成的信号网络。
Lateral organ boundaries domain (LBD)/ASL (AS2-like) genes encode plant–specific transcription factors and play important roles in plant growth and development. However, little information is available about theLBD15gene inArabidopsis. Here, the expression patterns of theLBD15gene were evaluated and its physiological roles inArabidopsiswere investigated. TheLBD15gene was expressed at different levels in many tissues including shoots, stems, roots, flowers and siliques. The overexpression ofLBD15can activate the transcription ofBOP1andBOP2, which are critical to controlling lateral organ development and inflorescence architecture. In addition, our results indicate that SND1, which is a key regulator of secondary wall synthesis, can bind to specific SNBE elements of theLBD15promoter to activate the expression ofLBD15. Elevated expression ofLBD15was found to down-regulate the transcription ofCesA4,CesA7, andCesA8, which encode cellulose synthesis of the secondary cell wall, and disrupt the secondary cell wall formation. In this way,LBD15may be involved in regulating secondary cell wall synthesis during differentiation of xylem cells. The present study provides novel evidence that may facilitate understanding the signaling network of lateral branch development and secondary cell wall synthesis inArabidopsis.