3'-TERMINAL AND 5'-TERMINAL SEQUENCES OF INFLUENZA-A, INFLUENZA-B AND INFLUENZA-C VIRUS-RNA SEGMENTS ARE HIGHLY CONSERVED AND SHOW PARTIAL INVERTED COMPLEMENTARITY
3'-TERMINAL AND 5'-TERMINAL SEQUENCES OF INFLUENZA-A, INFLUENZA-B AND INFLUENZA-C VIRUS-RNA SEGMENTS ARE HIGHLY CONSERVED AND SHOW PARTIAL INVERTED COMPLEMENTARITY
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DOI：
10.1016/0378-1119(80)90007-4
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发表时间：
1980-01-01
期刊：
影响因子：
3.5
通讯作者：
PALESE, P
中科院分区：
文献类型：
--
作者：
DESSELBERGER, U;RACANIELLO, VR;PALESE, P
通过使用2种不同的测序技术对病毒RNA直接测序，鉴定甲型、B和丙型流感病毒基因组片段的3“-和5”-末端核苷酸。在各病毒的基因组片段之间以及在3种不同病毒类型的片段之间观察到3“末端和5”末端的高度保守性。在每个片段的5“端的位置17至22观察到尿苷富集区。保守的3“和5”-末端序列显示部分和反向互补。这一特征导致正链和负链RNA的3“末端序列非常相似，也可能使流感病毒的单链RNA形成锅柄结构。反向互补重复序列可能在病毒RNA复制的起始中起重要作用。
The 3''- and 5''-terminal nucleotides of the genome segments of an influenza A, B and C virus were identified by directly sequencing viral RNA using 2 different sequencing techniques. A high degree of conservation at the 3'' ends and at 5'' ends was observed among the genome segments of each virus and among the segments of the 3 different virus types. A uridine-rich region was observed from positions 17 through 22 at the 5'' end of each segment. The conserved 3'' and 5''-terminal sequences showed partial and inverted complementarity. This feature results in very similar sequences at the 3'' ends of the plus and minus strand RNA and may also enable single-strand RNA of influenza virus to form panhandle structures. Inverted complementary repeats may play an important role in initiation of viral RNA replication.