Adaptive optics for fluorescence wide-field microscopy using spectrally independent guide star and markers.
Adaptive optics for fluorescence wide-field microscopy using spectrally independent guide star and markers.
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DOI：
10.1117/1.3603847
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发表时间：
2011-07
影响因子：
3.5
通讯作者：
P. Vermeulen;E. Muro;T. Pons;V. Loriette;A. Fragola
中科院分区：
文献类型：
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作者：
P. Vermeulen;E. Muro;T. Pons;V. Loriette;A. Fragola
我们描述了商用宽视场显微镜成像路径中自适应光学环路的实现和使用。我们证明，当通过异常的生物样本进行成像时，可以保持原始显微镜的光学性能。用于照亮自适应光学环路的光源在激发和发射方面与样品光谱无关，因此它们不会出现在最终图像中，并且它们的使用不会导致样品漂白。将结果与使用没有自适应光学系统的相同显微镜获得的等效图像进行比较。
We describe the implementation and use of an adaptive optics loop in the imaging path of a commercial wide field microscope. We show that it is possible to maintain the optical performances of the original microscope when imaging through aberrant biological samples. The sources used for illuminating the adaptive optics loop are spectrally independent, in excitation and emission, from the sample, so they do not appear in the final image, and their use does not contribute to the sample bleaching. Results are compared with equivalent images obtained with an identical microscope devoid of adaptive optics system.