Archaeal type IIIRuBisCOs function in a pathway for AMP metabolism
Archaeal type IIIRuBisCOs function in a pathway for AMP metabolism
复制标题
DOI：
10.1126/science.1135999
复制
发表时间：
2007-02-16
期刊：
影响因子：
56.9
通讯作者：
Imanaka, Tadayuki
中科院分区：
文献类型：
--
作者：
Sato, Takaaki;Atomi, Haruyuki;Imanaka, Tadayuki
在古细菌kodakaraensis中发现III型核酮糖-1,5-二磷酸羧化酶（RuBisCO）参与腺苷5'-单磷酸（AMP）代谢，其作用与经典的Calvin-Benson-Bassham循环中的RuBisCO不同。胸苷磷酸化酶（deoA）和真核翻译起始因子2B （e2b2）分别编码AMP磷酸化酶和核糖-1,5-二磷酸异构酶。这些酶从AMP和磷酸盐中提供RuBisCO底物1,5-二磷酸核酮糖。具有III型RuBisCOs的古细菌都含有DeoA和相应的E2b2同源物。在这一途径中，腺苷从AMP中释放出来，磷酸核糖部分进入中心碳代谢。
The type III ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) present in the archaeon Thermococcus kodakaraensis was found to participate in adenosine 5'-monophosphate ( AMP) metabolism, a role that is distinct from that of classical RuBisCOs of the Calvin-Benson-Bassham cycle. Genes annotated as thymidine phosphorylase (deoA) and eucaryal translation initiation factor 2B (e2b2) were found to encode AMP phosphorylase and ribose-1,5-bisphosphate isomerase, respectively. These enzymes supplied the RuBisCO substrate, ribulose-1,5-bisphosphate, from AMP and phosphate. Archaea with type III RuBisCOs all harbor both DeoA and the corresponding E2b2 homologs. In this pathway, adenine was released from AMP and the phosphoribose moiety entered central-carbon metabolism.