The Schizosaccharomyces pombe rhp3+ gene required for DNA repair and cell viability is functionally interchangeable with the RAD3 gene of Saccharomyces cerevisiae.
The Schizosaccharomyces pombe rhp3+ gene required for DNA repair and cell viability is functionally interchangeable with the RAD3 gene of Saccharomyces cerevisiae.
复制标题
DNA 修复和细胞活力所需的裂殖酵母 rhp3 基因在功能上可与酿酒酵母的 RAD3 基因互换。
DOI：
10.1093/nar/20.9.2327
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发表时间：
1992
影响因子：
14.9
通讯作者：
Prakash,S
中科院分区：
文献类型：
--
作者：
Reynolds,PR;Biggar,S;Prakash,L;Prakash,S
酵母中的RAD 3基因是细胞切除修复所必需的，其编码的蛋白具有单链DNA依赖的ATP酶、DNA解旋酶和DNA-RNA解旋酶活性。突变研究表明，在切除修复中需要RAD 3解旋酶活性。为了研究RAD 3在真核生物进化过程中结构和功能的保守程度，我们从亲缘关系较远的酵母裂殖酵母中克隆了RAD 3的同源基因rhp 3+，RAD 3和rhp 3+编码的蛋白高度相似，共有67%的相同氨基酸。我们发现，像RAD 3，rhp 3+是切除修复和细胞活力不可或缺的，我们的研究表明，在DNA修复的推定rhp 3 +DNA解旋酶活性的要求。我们发现RAD 3和rhp 3+基因在功能上可以相互替代。在S.pomberhp3突变体中由therhp 3+基因或由theRAD 3基因提供的互补水平是显著的，因为两种酵母中的切除修复和活力缺陷都恢复到野生型水平。这些观察结果表明，与RAD 3相互作用的其他蛋白质组分在介导其DNA修复和活力功能方面具有平行的进化保守性。
TheRAD3gene ofSaccharomyces cerevisiaeis required for excision repair and is essential for cell viability.RAD3encoded protein possesses a single stranded DNA-dependent ATPase and DNA and DNA-RNA helicase activities. Mutational studies have indicated a requirement for the RAD3 helicase activities in excision repair. To examine the extent of conservation of structure and function of RAD3 during eukaryotic evolution, we have cloned theRAD3homolog,rhp3+from the distantly related yeastSchizosaccharomyces pombe.RAD3andrhp3+encoded proteins are highly similar, sharing 67% identical amino acids. We show that likeRAD3,rhp3+is indispensable for excision repair and cell viability, and our studies indicate a requirement of the putative rhp3+DNA helicase activity in DNA repair. We find that theRAD3andrhp3+genes can functionally substitute for one another. The level of complementation provided by therhp3+gene in S.cerevisiaerad3mutants or by theRAD3gene in S.pomberhp3mutants is remarkable in that both the excision repair and viability defects in both yeasts are restored to wild type levels. These observations suggest a parallel evolutionary conservation of other protein components with which RAD3 interacts in mediating its DNA repair and viability functions.