An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils
An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils
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DOI：
10.1096/fj.202000289r
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发表时间：
2020-05-25
期刊：
影响因子：
4.8
通讯作者：
Menko, A. Sue
中科院分区：
文献类型：
--
作者：
DeDreu, JodiRae;Bowen, Caitlin J.;Menko, A. Sue
晶状体和中央角膜无血管。假设成人晶状体没有免疫细胞来源，晶状体周围的基底膜囊是免疫细胞迁移的屏障。然而，富含微原纤维相关蛋白-1 （MAGP1）的睫状体小带起源于富含血管的睫状体，并沿着晶状体囊表面延伸，形成了免疫细胞进入晶状体的潜在通道。在角膜清创损伤的反应中，我们发现MAGP1在整个角膜中央基质中的表达增加。在受伤后，这种典型的无血管区域的免疫细胞与这种富含magp1的基质密切相关。这些结果表明，富含magp1的微原纤维支持免疫细胞损伤后的迁移。利用该角膜创伤模型，我们研究了角膜损伤后是否存在涉及晶状体相关的富含magp1的睫状体带的免疫反应。我们的研究结果提供了第一个证据，证明角膜损伤后免疫细胞被激活，沿着晶状体赤道表面延伸的带状纤维移动，从那里它们迁移穿过晶状体前囊。这些结果表明，晶状体相关的睫状体带直接参与晶状体免疫反应，并提示睫状体是无血管晶状体免疫细胞的来源。
The lens and central cornea are avascular. It was assumed that the adult lens had no source of immune cells and that the basement membrane capsule surrounding the lens was a barrier to immune cell migration. Yet, microfibril-associated protein-1 (MAGP1)-rich ciliary zonules that originate from the vasculature-rich ciliary body and extend along the surface of the lens capsule, form a potential conduit for immune cells to the lens. In response to cornea debridement wounding, we find increased expression of MAGP1 throughout the central corneal stroma. The immune cells that populate this typically avascular region after wounding closely associate with this MAGP1-rich matrix. These results suggest that MAGP1-rich microfibrils support immune cell migration post-injury. Using this cornea wound model, we investigated whether there is an immune response to the lens following cornea injury involving the lens-associated MAGP1-rich ciliary zonules. Our results provide the first evidence that following corneal wounding immune cells are activated to travel along zonule fibers that extend anteriorly along the equatorial surface of the lens, from where they migrate across the anterior lens capsule. These results demonstrate that lens-associated ciliary zonules are directly involved in the lens immune response and suggest the ciliary body as a source of immune cells to the avascular lens.