Immunocytochemical localization of D1 and D2 dopamine receptors in the basal ganglia of the rat: Light and electron microscopy
Immunocytochemical localization of D1 and D2 dopamine receptors in the basal ganglia of the rat: Light and electron microscopy
复制标题
DOI：
10.1016/0306-4522(94)00536-e
复制
发表时间：
1995-04
期刊：
影响因子：
3.3
通讯作者：
K. Yung;J. Bolam;Anthony Smith;S. Hersch;B. Ciliax;A. Levey
中科院分区：
文献类型：
--
作者：
K. Yung;J. Bolam;Anthony Smith;S. Hersch;B. Ciliax;A. Levey
多巴胺对通过基底神经节的皮质信息流的调节作用主要通过两种受体亚型介导，即D1和D2受体。为了检查这些受体的精确的细胞和亚细胞位置，使用亚型特异性抗体的免疫细胞化学进行大鼠基底神经节的光镜和电镜水平的部分。过氧化物酶法和包埋前免疫金法均被采用。D1和D2受体的免疫反应性是最丰富的新纹状体，它主要是在多刺树突和胞体。虽然一些免疫反应阳性的胞体具有中间神经元的特征，但大多数被鉴定为中等大小的棘状神经元。D1受体的免疫反应性与纹状体黑质通路的轴突和黑质网状部和脚内核的轴突和终末相关，而与D2受体无关。与此相反，D2免疫反应，但不D1免疫反应存在于多巴胺能神经元的黑质部延髓腹侧和网状部。在苍白球，无论是D1或D2受体的免疫反应性检测很少。在亚细胞水平，D1和D2受体免疫反应主要与细胞膜的内表面有关。在树突和棘中，免疫反应性被认为是接触突触后膜的终端形成对称的突触和不太常见的，不对称的突触。形成对称突触的终端的形态特征和膜专业化是类似的多巴胺能终端先前确定的酪氨酸羟化酶的免疫细胞化学。除了与突触相关的免疫反应性外，高比例的免疫反应性也在非突触部位的膜上。它的结论是，多巴胺受体免疫反应性主要与多刺输出神经元的新纹状体，并有一个选择性协会的D1受体与所谓的直接途径的信息流通过基底神经节，即纹状体脚和纹状体黑质途径。虽然有一个协会的受体免疫反应性与传入突触输入的高比例位于突触外网站。
The modulatory actions of dopamine on the flow of cortical information through the basal ganglia are mediated mainly through two subtypes of receptors, the D1and D2receptors. In order to examine the precise cellular and subcellular location of these receptors, immunocytochemistry using subtype specific antibodies was performed on sections of rat basal ganglia at both the light and electron microscopic levels. Both peroxidase and pre-embedding immunogold methods were utilized. Immunoreactivity for both D1and D2receptors was most abundant in the neostriatum where it was mainly contained within spiny dendrites and in perikarya. Although some of the immunoreactive perikarya had characteristics of interneurons, most were identified as medium-sized spiny neurons. Immunoreactivity for D1receptor but not D2receptor was associated with the axons of the striatonigral pathway and axons and terminals in the substantia nigra pars reticulata and the entopeduncular nucleus. In contrast, D2immunoreactivity but not D1immunoreactivity was present in the dopaminergic neurons in the substantia nigra pars compacta and ventral pars reticulata. In the globus pallidus, little immunoreactivity for either D1or D2receptor was detected. At the subcellular level, D1and D2receptor immunoreactivity was found to be mainly associated with the internal surface of cell membranes. In dendrites and spines immunoreactivity was seen in contact with the membranes postsynaptic to terminals forming symmetrical synapses and less commonly, asymmetrical synapses. The morphological features and membrane specializations of the terminals forming symmetrical synapses are similar to those of dopaminergic terminals previously identified by immunocytochemistry for tyrosine hydroxylase. In addition to immunoreactivity associated with synapses, a high proportion of the immunoreactivity was also on membranes at non-synaptic sites. It is concluded that dopamine receptor immunoreactivity is mainly associated with spiny output neurons of the neostriatum and that there is a selective association of D1receptors with the so-called direct pathway of information flow through the basal ganglia, i.e. the striatoentopeduncular and striatonigral pathways. Although there is an association of receptor immunoreactivity with afferent synaptic inputs a high proportion is located at extrasynaptic sites.