Silencing of Mythimna separata chitinase genes via oral delivery of in planta-expressed RNAi effectors from a recombinant plant virus
Silencing of Mythimna separata chitinase genes via oral delivery of in planta-expressed RNAi effectors from a recombinant plant virus
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通过口服重组植物病毒在植物中表达的 RNAi 效应子来沉默粘虫几丁质酶基因。
DOI：
10.1007/s10529-016-2186-0
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发表时间：
2016-11-01
影响因子：
2.7
通讯作者：
Wuriyanghan, Hada
中科院分区：
文献类型：
--
作者：
Bao, Wenhua;Cao, Budao;Wuriyanghan, Hada
利用重组病毒载体在本氏烟草中瞬时表达RNA干扰（RNAi）效应子，并口服该效应子沉默粘虫（Mythimna separata）内源基因的表达。目的：评价靶向M.分离，我们克隆了M的片段。分离几丁质酶序列到病毒载体中，以便在植物中病毒感染和复制期间产生RNAi效应物。将感染的植株喂给M.分离的，目标MseChi1和MseChi2基因的表达水平分别下调了76%和45%，并在受体昆虫中检测到序列特异性siRNA。基于RNAi的几丁质酶基因沉默也导致体重下降43%。我们的研究表明，靶mRNA敲除，并提出了一个有前途的应用控制M。通过使用重组植物病毒在植物中表达RNAi效应子来分离分离植物。
To evaluate transient expression of RNA interference (RNAi) effectors in Nicotiana benthamiana plants by using recombinant virus vectors and also oral delivery of the effectors for silencing of Mythimna separata endogenous gene expression.Mythimna separata is a serious pest of corn production in China. To evaluate RNAi approaches to target specific RNAs in M. separate, we cloned fragments of the M. separata chitinase sequences into a virus vector in order to produce RNAi effectors during virus infection and replication in plants. When the infected plants were fed to M. separata, expression levels of target MseChi1 and MseChi2 genes were down-regulated by 76 and 45 %, respectively, and sequence-specific siRNAs were detected in recipient insects. RNAi-based silencing of chitinase genes also led to body weight decreases by 43 %.Our research demonstrates target mRNA knockdown and suggests a promising application for controlling of M. separata by in planta expression of RNAi effectors using a recombinant plant virus.