Oestrogen receptors alpha and beta differ in normal human breast and breast carcinomas
Oestrogen receptors alpha and beta differ in normal human breast and breast carcinomas
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DOI：
10.1002/path.1230
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发表时间：
2002-12-01
影响因子：
7.3
通讯作者：
Walker, RA
中科院分区：
文献类型：
--
作者：
Shaw, JA;Udokang, K;Walker, RA
第二种雌激素受体，雌激素受体(ER) β的鉴定，导致需要评估经典MY和erβ在人类乳腺癌和乳腺癌中的相对重要性。应用逆转录酶巢式聚合酶链式反应（RT）对61例乳腺癌、8例乳腺良性病变和30例正常乳腺标本中的erα和erβ mRNA进行了检测。对62例癌组织、38例非恶性乳腺组织和32例有月经周期资料的正常乳腺组织进行erα和erβ的免疫组化分析。在9217'c的乳腺癌中检测到Me mRNA， 85%的乳腺癌中检测到ER mRNA（野生型和/或变异型）；72%的人有ERa蛋白，62%的人有孕激素受体（PgR）， 32%的人有erβ。erα蛋白与肿瘤分级有很强的相关性，erβ蛋白则没有，尽管它存在于4个1级肿瘤中的3个和特殊类型中。erα和erβ蛋白的存在之间没有相关性。在非恶性乳腺中，除erβ在基质细胞和肌上皮中表达外，erα和β表达相似，后者通过RT-PCR和western blotting证实。erα与月经周期有差异，而PgR和erβ无差异。研究结果表明，有必要了解ERbeta在正常乳腺中的作用和调节，以及其在乳腺癌发生中的下调原因。版权所有:John Wiley Sons, Ltd。
The identification of a second oestrogen receptor, oestrogen receptor (ER) beta, has led to a need to assess the relative importance of the classical MY and ERbeta in human breast and breast carcinomas. ERalpha and ERbeta mRNA was assessed in 61 carcinomas, 8 benign breast lesions, and 30 samples of normal breast using reverse transcriptase (RT)-nested polymerase chain reaction (PCR). Immunohistochemical analysis of ERalpha and ERbeta was performed in 62 carcinomas, the 38 non-malignant breast tissues, and 32 normal breast samples with menstrual cycle data. Me mRNA was detected in 9217'c of breast cancers, with ER mRNA (wild-type and/or variant form) in 85%; 72% had ERa protein, 62% progesterone receptor (PgR), and 32% ERbeta. ERalpha protein had a strong correlation sitli grade, ERbeta did not, although it was present in three of four grade 1 carcinomas and in special types. There was no correlation between the presence of ERalpha and ERbeta protein. In non-malignant breast, similar expression of ERalpha and beta was observed, apart from expression of ERbeta in stromal cells and myoepithelium, the latter being confirmed by RT-PCR and western blotting. There were differences in ERalpha in relation to the menstrual cycle but not PgR or ERbeta. The findings indicate a need to understand the role and regulation of ERbeta in normal breast and the reason for its down-reguIation in mammary carcinogenesis. Copyright (C) 2002 John Wiley Sons, Ltd.