First Evidence of the Presence of Anatoxin-A in Sea Figs Associated with Human Food Poisonings in France
First Evidence of the Presence of Anatoxin-A in Sea Figs Associated with Human Food Poisonings in France
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DOI：
10.3390/md18060285
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发表时间：
2020-06-01
期刊：
影响因子：
5.4
通讯作者：
Nicolas, Marina
中科院分区：
文献类型：
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作者：
Bire, Ronel;Bertin, Thomas;Nicolas, Marina
2011年1月至2018年3月，法国有26名年龄在20岁至80岁之间的患者报告在食用了microcosmus属海无花果后患病。患者有引起小脑综合征的症状：视力模糊或重影、共济失调和头晕、虚弱、头痛、肌肉痉挛、感觉异常和消化系统紊乱（恶心、呕吐和腹泻）。在马赛毒物控制中心记录的18起食物中毒事件中，有3起涉及4名患者，并对剩菜剩饭进行了收集和分析。先前的研究在高分辨率质谱分析后排除了受调节的亲脂性海洋毒素的存在，但需要进一步的分析来寻找亲水的蓝藻毒素。本文采用亲水性相互作用液相色谱-低分辨率和高分辨率质谱联用技术，对已发表的病例系列中的1号（2011年1月）、6号（2012年12月）和17号（2018年3月）食物中毒病例中的海无花果剩余物进行分析，以调查亲水性蓝藻毒素的存在。海无花果样品中anatoxin-a （ATX-a）的浓度为193.7 ~ 1240.2 μ g/kg。对照样品也被ATX-a污染，但浓度要小得多（22.5 μ g/kg）。据我们所知，这是人类食物中毒的第一份报告，涉及ATX-a作为可能的致病毒素，而蓝藻毒素可以明确识别。
From January 2011 to March 2018, 26 patients aged from 20 to 80 years old reported being sick in France after eating sea figs of the genusMicrocosmus. The patients had symptoms evoking a cerebellar syndrome: blurred or double vision, ataxia and dizziness, asthenia, headache, muscle cramps, paresthesia and digestive disorders (nausea, vomiting and diarrhea). Three of the 18 food poisoning events recorded by the Poison Control Center in Marseille and involving four patients were further investigated as the meal leftovers were collected and analyzed. A previous study ruled out the presence of the regulated lipophilic marine toxins after high-resolution mass spectrometry, but further analyses were required to look for hydrophilic cyanotoxins. The sea fig leftovers from food poisoning case Numbers 1 (January 2011), 6 (December 2012) and 17 (March 2018) of this published case series were analyzed by hydrophilic interaction liquid chromatography coupled to low- and high-resolution mass spectrometry to investigate the presence of hydrophilic cyanotoxins. The sea fig samples showed anatoxin-a (ATX-a) concentrations ranging from 193.7 to 1240.2 mu g/kg. The sea fig control sample analyzed was also contaminated with ATX-a but in a much smaller concentration (22.5 mu g/kg). To the best of our knowledge, this is the first report of human food poisoning involving ATX-a as the possible causative toxin where the cyanotoxin could be unequivocally identified.