Real-time chemical measurements of dopamine release in the brain.
Real-time chemical measurements of dopamine release in the brain.
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DOI：
10.1007/978-1-62703-251-3_16
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Sombers, Leslie A
中科院分区：
文献类型：
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作者：
Roberts, James G;Lugo-Morales, Leyda Z;Sombers, Leslie A
用快速扫描循环伏安法(FSCV)可以检测到自由活动或麻醉大鼠细胞外多巴胺浓度的快速变化。背景减去FSCV是一种实时电化学技术，它可以在亚秒的时间尺度上监测大脑中的神经化学传递，同时提供有关分析物的化学信息。此外，这种伏安法还允许研究大脑中分子的释放和摄取的动力学。本章完整地描述了如何进行这些测量以及FSCV的特性，使其独特地适合于在体内进行多巴胺能神经传递的化学测量。
Rapid changes in extracellular dopamine concentrations in freely moving or anesthetized rats can be detected using fast-scan cyclic voltammetry (FSCV). Background-subtracted FSCV is a real-time electrochemical technique that can monitor neurochemical transmission in the brain on a subsecond timescale, while providing chemical information on the analyte. Also, this voltammetric approach allows for the investigation of the kinetics of release and uptake of molecules in the brain. This chapter describes, completely, how to make these measurements and the properties of FSCV that make it uniquely suitable for performing chemical measurements of dopaminergic neurotransmission in vivo.