Differential calbindin-like immunoreactivity in the brain stem auditory system of the chinchilla.
Differential calbindin-like immunoreactivity in the brain stem auditory system of the chinchilla.
复制标题
龙猫脑干听觉系统中的钙结合蛋白样免疫反应性差异。
DOI：
10.1002/cne.903200205
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Walton,JP
中科院分区：
文献类型：
--
作者：
Kelley,PE;Frisina,RD;Zettel,ML;Walton,JP
钙结合蛋白是一种在神经组织中发现的28 kD钙结合蛋白。虽然其在神经元中的功能作用是未知的，它已被提出，钙结合蛋白参与细胞内缓冲，因此可能会影响神经元放电的时间精度。在仓鸮中，钙结合蛋白样免疫反应性被发现选择性地存在于用于处理耳间时间差的脑干听觉通路中，但不存在于耳间强度通路中。本研究证明了在灰鼠的听觉脑干中的钙结合蛋白免疫反应性，灰鼠是一种具有非常好的低频听力的啮齿动物。在上级橄榄复合体和橄榄周区，免疫反应分为外侧和内侧上级橄榄和背内侧橄榄周核的神经元标记，以及斜方体的内侧和腹侧核和前外侧橄榄周核的胞体的标记。在外侧丘系腹侧核的腹侧和背侧部以及腹侧部的柱状纤维丛中观察到强免疫反应。外侧丘系背核无免疫反应。几乎所有的主要神经元的sagulum显示暗标记的胞体周围的密集标记的纤维丛。下丘的免疫反应主要局限于中央旁核，只有偶尔在中央核的标记细胞。总之，虽然在哺乳动物听觉脑干钙结合蛋白的选择性标记是令人印象深刻的，没有明显的功能性的定时通路的独特的标签，如先前报道的谷仓猫头鹰或电鱼。
Calbindin is a 28 kD calcium‐binding protein found in neural tissue. Although its functional role in neurons is unknown, it has been proposed that calbindin is involved in intracellular buffering and could therefore influence temporal precision of neuronal firing. In the barn owl, calbindin‐like immunoreactivity was found to be selectively present in brain stem auditory pathways used to process interaural time differences, but was absent from the interaural intensity pathway. The present study demonstrates calbindin immunoreactivity in the auditory brain stem of the chinchilla, a rodent with exceptionally good low‐frequency hearing. In the superior olivary complex and periolivary areas, immunoreactivity was divided between neuropil labeling in the lateral and medial superior olives and dorsomedial periolivary nucleus, and labeling of the somata of the medial and ventral nuclei of the trapezoid body and anterolateral periolivary nucleus. Strong immunoreactivity was observed in the ventral and dorsal divisions of the ventral nucleus of lateral lemniscus somata and the vental division's columnarly organized fiber plexus. The dorsal nucleus of the lateral lemniscus was void of immunoreactivity. Virtually all principal neurons of the sagulum showed darkly labeled somata surrounded by a densely labeled fiber plexus. Immunoreactivity in the inferior colliculus was primarily limited to the paracentral nuclei, with only an occasional labeled cell in the central nucleus. In conclusion, although selective labeling of calbindin in the mammalian auditory brain stem is impressive, no distinctive labeling of a functionally denned timing pathway was apparent as reported previously in the barn owl or electric fish.