Tumor-targeted Graphitic Carbon Nitride Nanoassembly for Activatable Two-photon Fluorescence Imaging
Tumor-targeted Graphitic Carbon Nitride Nanoassembly for Activatable Two-photon Fluorescence Imaging
复制标题
用于可激活双光子荧光成像的肿瘤靶向石墨氮化碳纳米组件
DOI：
10.1021/acs.analchem.7b05192
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发表时间：
2018
影响因子：
7.4
通讯作者：
Jian-Hui Jiang
中科院分区：
文献类型：
--
作者：
Jin-Wen Liu;Yu-Min Wang;Chong-Hua Zhang;Lu-Ying Duan;Zheng Li;Ru-Qin Yu;Jian-Hui Jiang
石墨氮化碳（g-CN）纳米片独特的物理化学特征使其成为双光子荧光生物成像的有用工具。目前的基于g-CN纳米片的成像探针通常使用“始终开启”的设计策略，其可能遭受增加的荧光背景和有限的对比度。为了推进相应的应用，基于g-CN纳米片的可活化双光子荧光探针仍有待于探索。我们首次开发了一种可激活的双光子荧光探针，由g-CN纳米片和透明质酸（HA）-金纳米颗粒（HA-AuNPs）的纳米组装体构建，用于检测和成像癌细胞中的透明质酸酶（HAase）。在我们的设计中故意引入的HA不仅用作稳定AuNP和诱导g-CN纳米片上相应自组装的缓冲层，而且还用作靶向癌细胞表面上过表达的HA受体的先导。我们的研究结果表明，所开发的纳米组装能够特异性检测和激活癌细胞和深部组织中的HAase成像，具有极好的信号背景比和高灵敏度。这种纳米组装体可以为HAase的高度特异性和敏感性成像以及相关癌症诊断提供有前途的平台。
Unique physicochemical characteristics of graphitic carbon nitride (g-CN) nanosheets suit them to be a useful tool for two-photon fluorescence bioimaging. Current g-CN nanosheets based imaging probes typically use the “always-on” design strategies, which may suffer from increased fluorescence background and limited contrast. To advance corresponding applications, g-CN nanosheets based activatable two-photon fluorescence probes remain to be explored. For the first time, we developed an activatable two-photon fluorescence probe, constructed from a nanoassembly of g-CN nanosheets and hyaluronic acid (HA)–gold nanoparticles (HA–AuNPs), for detection and imaging of hyaluronidase (HAase) in cancer cells. The deliberately introduced HA in our design not only functions as the buffering layer for stabilizing AuNPs and inducing corresponding self-assembly on g-CN nanosheets but also as a pilot for targeting HA receptors overexpressed on cancer cell surfaces. Our results show that the developed nanoassembly enables specific detection and activatable imaging of HAase in cancer cells and deep tissues, with superb signal-to-background ratio and high sensitivity. This nanoassembly can afford a promising platform for highly specific and sensitive imaging of HAase and for related cancer diagnosis.