Antibody to a recombinant merozoite protein epitope identifies horses infected with Babesia equi
Antibody to a recombinant merozoite protein epitope identifies horses infected with Babesia equi
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重组裂殖子蛋白表位的抗体可识别感染马巴贝虫的马
DOI：
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发表时间：
1992
影响因子：
9.4
通讯作者：
L. E. Perryman
中科院分区：
文献类型：
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作者：
D. P. Knowles;Lowell S. Kappmeyer;David Stiller;S. Hennager;L. E. Perryman
先前通过与裂殖子表面蛋白表位反应的抗体的存在来鉴定感染马巴贝虫的马（D.P.Knowles，Jr.，L.E.Perryman，L.S.Kappmeyer，和S.G.Hennager.J.Clin.Microbiol.29：2056-2058，1991）。使用单克隆抗体 36/133.97 在竞争性抑制酶联免疫吸附测定 (CI ELISA) 中检测抗体，该抗体定义了裂殖子表面的蛋白质表位。编码该马芽孢杆菌裂殖子表位的基因被克隆并在大肠杆菌中表达。重组裂殖子蛋白，称为等裂殖子抗原 1 (EMA-1)，在 CI ELISA 中进行评估。重组 EMA-1 结合抗体来自来自 18 个国家的马伯伯氏菌感染的马的血清。然后在通过蜱载体微小牛蜱传播或通过静脉内接种而实验性感染马双歧杆菌的马中测量对 EMA-1 的抗体反应。抗 EMA-1 抗体在蜱暴露后 7 周被检测到，并在评估期的 33 周内保持不变，没有再次暴露于马蜱。数据表明重组 EMA-1 可用于 CI ELISA 检测感染马双歧杆菌的马。
Horses infected with Babesia equi were previously identified by the presence of antibodies reactive with a merozoite surface protein epitope (D. P. Knowles, Jr., L. E. Perryman, L. S. Kappmeyer, and S. G. Hennager. J. Clin. Microbiol. 29:2056-2058, 1991). The antibodies were detected in a competitive inhibition enzyme-linked immunosorbent assay (CI ELISA) by using monoclonal antibody 36/133.97, which defines a protein epitope on the merozoite surface. The gene encoding this B. equi merozoite epitope was cloned and expressed in Escherichia coli. The recombinant merozoite protein, designated equi merozoite antigen 1 (EMA-1), was evaluated in the CI ELISA. Recombinant EMA-1 bound antibody from the sera of B. equi-infected horses from 18 countries. The antibody response to EMA-1 was then measured in horses experimentally infected with B. equi via transmission by the tick vector Boophilus microplus or by intravenous inoculation. Anti-EMA-1 antibody was detected 7 weeks post-tick exposure and remained, without reexposure to B. equi, for the 33 weeks of the evaluation period. The data indicate that recombinant EMA-1 can be used in the CI ELISA to detect horses infected with B. equi.
DOI：
10.1073/pnas.80.5.1194
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
YOUNG, RA;DAVIS, RW
通讯作者：
DAVIS, RW