Diurnal variation of circulating microvesicles is associated with the severity of obstructive sleep apnoea
Diurnal variation of circulating microvesicles is associated with the severity of obstructive sleep apnoea
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DOI：
10.1007/s11325-017-1464-y
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发表时间：
2017-09-01
影响因子：
2.5
通讯作者：
Komlosi, Zsolt Istvan
中科院分区：
文献类型：
--
作者：
Bikov, Andras;Kunos, Laszlo;Komlosi, Zsolt Istvan
目的微泡（MV）与阻塞性睡眠呼吸暂停（OSA）的病理机制有关，但目前的研究结果并不一致，可能是由于循环MV水平的时间变化未被揭示。方法18例OSA患者和9例健康对照者分别于11：00、17：00、21：00、01：30和06：00采集外周血，测定MV各组分的日变化。在持续气道正压通气（CPAP）治疗2个月后，对9名OSA患者重复采样。流式细胞术检测CD41+、CD62 P+、血型糖蛋白A+和Annexin V+的MV。结果血浆CD 41+和Annexin V+ MV浓度在OSA患者中存在明显的昼夜变化，峰值出现在17：00。CD41+ MVAUC与OSA的严重程度直接相关。结论CD41+ MV的昼夜变化与疾病严重程度之间的关系以及CPAP治疗对MV水平的影响支持MV在OSA的病理生理学中的作用。更重要的是，考虑到循环MV水平的显著昼夜变化，MV测量需要引入严格的血液采样方案。
Purpose Microvesicles (MVs) have been implicated in the pathomechanism of obstructive sleep apnoea (OSA); however, the results are inconsistent, possibly due to an unrevealed temporal variation in circulating MV levels. We aimed to investigate the diurnal changes of MV fractions in OSA.Methods Peripheral blood was taken from 18 patients with OSA and 9 healthy subjects at different time points (11: 00, 17: 00, 21: 00, 01: 30 and 06: 00). Samplings were repeated in nine OSA patients after 2 months of continuous positive airway pressure (CPAP) therapy. CD41+, CD62P+, glycophorin A+ and Annexin V+ MVs were determined with flow cytometry. Areas under the MV concentrations-time curves (AUC) were calculated and correlated with the severity of OSA.Results A significant diurnal variability of plasma CD41+ and Annexin V+ MVs was observed only in OSA with a marked peak at 17: 00. There was a direct correlation between CD41+ MVAUCs and the severity of OSA. CPAP treatment reduced diurnal variability in both CD41+ and Annexin V+ MV levels.Conclusions The relationship between the diurnal variability of CD41+ MVs and disease severity as well as the effect of CPAP treatment on MV levels support the role of MVs in the pathophysiology of OSA. More importantly, considering the significant diurnal variation in circulating MV levels, introduction of strict protocols for blood sampling is required for MV measurements.