Genetic Variability of Hepatitis C Virus in South Egypt and Its Possible Clinical Implication
Genetic Variability of Hepatitis C Virus in South Egypt and Its Possible Clinical Implication
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DOI：
10.1002/jmv.21492
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发表时间：
2009-06-01
影响因子：
12.7
通讯作者：
Mizokami, Masashi
中科院分区：
文献类型：
--
作者：
Elkady, Abeer;Tanaka, Yasuhito;Mizokami, Masashi
埃及是丙型肝炎病毒相关发病率和死亡率非常高的国家之一。然而，埃及人对丙型肝炎病毒基因变异性的地理和临床差异知之甚少。利用直接测序和对丙型肝炎病毒基因组部分核心/E1和NS5B区域的系统发育分析，对居住在埃及南部三个省的129名丙型肝炎病毒感染者进行了丙型肝炎病毒基因型/亚型的确定：阿苏特、索哈格和奥埃纳省。根据感染的临床分期，将患者分为4组：无症状携带者组16例，慢性丙型肝炎组36例，肝硬化组54例，肝细胞癌组23例。4a型占80.6%，1g、4l、4n、4o、4f和4m分别占7.7%、4.7%、3.9%、1.6%、0.8%和0.8%。4a的患病率在不同地区有所不同，从88.5%(索哈市)到%(阿西特省，P=0.002)。41和4N型在Assue中的患病率(12.8%，10.3%)高于Sohag(0%，0%；P<；0.011)。不同4a变异体(4l和4n、4f或4m)携带者的慢性肝炎发生率高于4a变异体携带者(53.3%比23.1%，P=0.025)，而4a变异体携带者(45.2%比13.3%，P=0.023)有更多的慢性肝炎。在这项研究中分离到两株丙型肝炎病毒，都是从肝细胞癌患者身上分离出来的。总之，这项研究揭示了埃及南部地区丙型肝炎病毒的地理多样性。这项研究表明，需要进一步的病例对照研究来确认丙型肝炎病毒亚型的不同致病性的趋势。J.Med.维罗尔。81：1015-1023,2009。(C)2009年Wiley-Liss Inc.
Egypt is one of the countries with very high rates of hepatitis C virus (HCV) related morbidity and mortality. However, little is known about geographical and clinical differences in genetic variability of HCV in Egypt. Using direct sequencing and phylogenetic analysis of partial core/E1 and NS5B regions of the HCV genome, HCV genotype/subtype was determined in 129 HCV-infected patients residing in three governates in south Egypt: Assuit, Sohag, and Oena. According to clinical stage of infection, patients were categorized into four groups: asymptornatic carriers, n = 16; chronic hepatitis C patients, n = 36; liver cirrhosis, n = 54; and hepatocellular carcinoma (HCC), n = 23. Genotype 4a was detected in 80.6%, whereas 1g, 4l, 4n, 4o, 4f, and 4m were identified in 7.7%, 4.7%, 3.9%, 1.6%, 0.8%, and 0.8% of cases, respectively. The prevalence of 4a differed regionally; from 88.5% (in Sohag) to 64% (in Assuit, P = 0.002). Genotypes 41 and 4n had a higher prevalence in Assuit (12.8%, 10.3%) than Sohag (0%, 0%; P < 0.011). Difference in clinical features of determined genotypes/subtypes was observed; more carriers of non-4a variants (4l and 4n, 4f, or 4m) had chronic hepatitis compared to carriers of 4a (53.3% vs. 23.1%, P = 0.025), while more patients with 4a had liver cirrhosis (45.2% vs. 13.3%, P = 0.023). Two HCV-4o strains were isolated in this study, both from patients with HCC. In conclusion, geographical diversity of HCV was revealed in this study in southern Egypt. A further case-control study is required to confirm the trends of differential pathogenicity of HCV subtypes, indicated by this study. J. Med. Virol. 81:1015-1023, 2009. (c) 2009 Wiley-Liss Inc.