Effects of a high-molecular-weight cranberry fraction on growth, biofilm formation and adherence of Porphyromonas gingivalis
Effects of a high-molecular-weight cranberry fraction on growth, biofilm formation and adherence of Porphyromonas gingivalis
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DOI：
10.1093/jac/dkl220
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发表时间：
2006-08-01
影响因子：
5.2
通讯作者：
Grenier, Daniel
中科院分区：
文献类型：
--
作者：
Labrecque, Julie;Bodet, Charles;Grenier, Daniel
背景：牙龈卟啉单胞菌是牙周炎的主要病原菌，牙周炎是一种影响牙齿支持组织的破坏性疾病。近年来的研究表明，蔓越莓汁浓缩液中的高分子物质可以防止病原体附着在宿主组织上。目的：研究从蔓越莓汁浓缩液中制备的非透析性材料(NDM)对牙周炎假单胞菌生长、膜形成和黏附特性的影响。方法：采用聚苯乙烯微板法和扫描电子显微镜研究蔓越莓汁非透析物对牙周炎假单胞菌生物膜形成的影响。通过固定化哺乳动物蛋白的微孔板实验，评价了酸莓NDM对牙龈假单胞菌附着特性的影响。结果：结果表明，酸莓NDM对牙龈假单胞菌生物膜的形成有较强的抑制作用。然而，它对细菌的生长和生存能力没有影响。小红莓NDM还能显著阻止A牙龈菌附着在涂有I型胶原、纤维蛋白原或人血清的表面。结论：小红莓成分可能通过减少A牙龈菌在牙周部位的定植能力而起到预防和治疗牙周炎的作用。
Background: Porphyromonas gingivalis is a major aetiological agent of periodontitis, a destructive disease affecting the tooth-supporting tissues. Recent reports have indicated that high-molecular-weight molecules from cranberry juice concentrate can prevent the attachment of human pathogens to host tissues.Objectives: The aim of the present study was to investigate the effect of non-dialysable material (NDM) prepared from cranberry juice concentrate on growth, blofilm formation and adherence properties of P. gingivalis.Methods: The effect of cranberry NDM on biofilm formation was studied using a polystyrene microplate assay and by scanning electron microscopy. The effect of cranberry NDM on the attachment properties of P. gingivalis was evaluated by a microplate assay in which mammalian proteins were immobilized into wells.Results: Our results indicated that cranberry NDM is a potent inhibitor of biofilm formation by P. gingivalis. However, it has no effect on growth and viability of bacteria. Cranberry NDM also prevented significantly the attachment of A gingivalis to surfaces coated with type I collagen, fibrinogen or human serum.Conclusions: Our data suggest that cranberry constituents may have a beneficial effect for the prevention and treatment of periodontitis by reducing the capacity of A gingivalis to colonize periodontal sites.