Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells.
Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells.
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DOI：
10.1007/978-1-0716-0810-4_26
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Papapetrou EP
中科院分区：
文献类型：
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作者：
Deslauriers AG;Kotini AG;Papapetrou EP
患者来源的诱导多能干细胞（iPSC）最近提供了一种新的方法来模拟急性髓性白血病（AML）和其他髓系恶性肿瘤。在这里，我们描述了从白血病细胞产生患者来源的iPSC的方法，以及它们随后在体外定向分化成造血细胞的方法，所述造血细胞概括了白血病干细胞（LSC）和白血病母细胞的特征。
Patient-derived induced pluripotent stem cells (iPSCs) have recently provided a new way to model acute myeloid leukemia (AML) and other myeloid malignancies. Here, we describe methods for the generation of patient-derived iPSCs from leukemia cells and for their subsequent directed in vitro differentiation into hematopoietic cells that recapitulate features of leukemia stem cells (LSCs) and leukemic blasts.