A flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in piglets
A flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in piglets
复制标题
鞭毛蛋白佐剂灭活猪流行性腹泻病毒 (PEDV) 疫苗可增强仔猪针对 PEDV 攻击的免疫保护
DOI：
10.1007/s00705-020-04567-w
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发表时间：
2020
影响因子：
2.7
通讯作者：
Bin Li
中科院分区：
文献类型：
--
作者：
Xiangwei Xu;Luping Du;Baochao Fan;Bing Sun;Jinzhu Zhou;Rongli Guo;Zhengyu Yu;Danyi Shi;Kongwang He;Bin Li
自2010年底以来，中国养猪业爆发了猪流行性腹泻（PED）。一种不同于CV777的PEDV变种毒株引起流行的PEDV感染，而基于CV777的商业疫苗不能提供完全的保护。在这项研究中，我们设计了一种基于流行PEDV菌株AH2012/12的新疫苗，辅以鞭毛蛋白（一种诱导粘膜和全身产生IgA的粘膜佐剂）。三组妊娠母猪在分娩前分别用鞭毛蛋白、PEDV单独或PBS佐剂接种PEDV，每隔14天接种两次，并从每组中选择新生仔猪接种PEDV。接种该疫苗可在母猪血清和初乳中产生高水平的IgG、IgA和中和抗体，新生仔猪在哺乳期间可免受PEDV感染。该研究将指导PEDV感染的预防和控制策略，从而减少与该病毒相关的损失。
Since late 2010, outbreaks of porcine epidemic diarrhea (PED) have been reported in the swine industry in China. A variant PEDV strain that differs from strain CV777 causes prevalent PEDV infections which commercial vaccines based on CV777 cannot provide complete protection. In this study, we designed a new vaccine based on the epidemic PEDV strain AH2012/12, adjuvanted with flagellin, a mucosal adjuvant that induces mucosal and systemic production of IgA. Three groups of pregnant sows were immunized twice, with a 14-day interval, with PEDV adjuvanted with flagellin, PEDV alone, or PBS before farrowing, and newborn piglets from each group were selected and challenged with PEDV. Immunization with this vaccine elicited high levels of IgG, IgA, and neutralizing antibodies in the serum and colostrum of sows, and newborn piglets were protected against PEDV while suckling. This study should guide the prevention and control strategies for PEDV infection, thereby reducing the losses associated with this virus.