EFFECTS OF SULFUR NUTRITION ON EXPRESSION OF THE SOYBEAN SEED STORAGE PROTEIN GENES IN TRANSGENIC PETUNIA
EFFECTS OF SULFUR NUTRITION ON EXPRESSION OF THE SOYBEAN SEED STORAGE PROTEIN GENES IN TRANSGENIC PETUNIA
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DOI：
10.1104/pp.99.1.263
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发表时间：
1992-05-01
期刊：
影响因子：
7.4
通讯作者：
NAITO, S
中科院分区：
文献类型：
--
作者：
FUJIWARA, T;HIRAI, MY;NAITO, S
大豆7S种子贮藏蛋白（β -conglycinin） [L]。)有三个主要的亚单位；'和。外源蛋氨酸可抑制未成熟子叶培养系统中β亚基的积累（LP Holowach, JF Thompson, JT Madison [1984] Plant Physiol 74: 576-583），而在硫酸盐缺乏的情况下，β亚基的积累会增加（KR Gayler, GE Sykes [1985] Plant Physiol 78: 582-585）。构建了含有α亚基或β亚基基因的转基因矮牵牛（petunia hybrida），以检验其差异表达模式是否在矮牵牛中保留。矮牵牛对这些基因的调节方式与大豆对硫营养刺激的反应类似，即(a)体外培养种子中β亚基基因的表达受到外源蛋氨酸的抑制，而α亚基基因的表达不受影响；(b)缺硫促进了β亚基的积累。矮牵牛主要种子贮藏蛋白的积累模式不受这些处理的影响。这些结果表明，即使不用于调节其自身主要的种子储存蛋白，这种响应硫营养的基因调控机制在矮牵牛中是保守的。
The 7S seed storage protein (beta-conglycinin) of soybean (Glycine max [L]. Merr.) has three major subunits; alpha, alpha', and beta. Accumulation of the beta-subunit, but not the alpha- and alpha'-subunits, has been shown to be repressed by exogenously applied methionine to the immature cotyledon culture system (LP Holowach, JF Thompson, JT Madison [1984] Plant Physiol 74: 576-583) and to be enhanced under sulfate deficiency in soybean plants (KR Gayler, GE Sykes [1985] Plant Physiol 78: 582-585). Transgenic petunia (Petunia hybrida) harboring either the alpha'- or beta-subunit gene were constructed to test whether the patterns of differential expression were retained in petunia. Petunia regulates these genes in a similar way as soybean in response to sulfur nutritional stimuli, i.e. (a) expression of the beta-subunit gene is repressed by exogenous methionine in in vitro cultured seeds, whereas the alpha'-subunit gene expression is not affected; and (b) accumulation of the beta-subunit is enhanced by sulfur deficiency. The pattern of accumulation of major seed storage protein of petunia was not affected by these treatments. These results indicate that this mechanism of gene regulation in response to sulfur nutrition is conserved in petunia even though it is not used to regulate its own major seed storage proteins.