Nanoparticle Ligand Exchange and Its Effects at the Nanoparticle-Cell Membrane Interface.
Nanoparticle Ligand Exchange and Its Effects at the Nanoparticle-Cell Membrane Interface.
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DOI：
10.1021/acs.nanolett.8b02638
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发表时间：
2018-10
期刊：
影响因子：
10.8
通讯作者：
Xinyi Wang;Xiaofeng Wang;X. Bai;Liang Yan;Tao Liu;Mingzhe Wang;Youtao Song;Guoqing Hu;
中科院分区：
文献类型：
--
作者：
Xinyi Wang;Xiaofeng Wang;X. Bai;Liang Yan;Tao Liu;Mingzhe Wang;Youtao Song;Guoqing Hu;
纳米颗粒（nano）-细胞膜界面是决定纳米颗粒（NP）命运的最重要的相互作用之一，它可以刺激一系列生物事件，从而允许治疗诊断和其他生物医学应用。到目前为止，人们仍然缺乏关于控制纳米颗粒-细胞膜界面的机制的了解，特别是配体交换的影响，其中纳米表面上的分子被细胞膜的成分取代，从而导致独特的界面现象。在此，我们描述了一系列具有相同核心尺寸（〜13 nm核心）的金纳米颗粒（AuNP），用12种不同的表面配体进行修饰，以及它们的可交换配体对纳米颗粒支持的脂质双层（SLB）和纳米颗粒-天然细胞膜界面的影响。配体根据其分子量、电荷和键合模式（物理吸附或化学吸附）进行分类。重要的是，我们发现，根据配体分子的吸附亲和力和大小，纳米颗粒表面的物理吸附配体可以与脂质分子交换。在配体交换主导的界面处，AuNP 通常聚集成脂质双层中的有序单层，随后影响细胞膜完整性、NP 摄取效率和 NP 内吞途径。这些发现促进了我们从新的角度理解纳米粒子生物效应的潜在机制，并将有助于设计新颖、安全、有效的生物医学纳米材料。
The nanoparticle (nano)-cell membrane interface is one of the most important interactions determining the fate of nanoparticles (NPs), which can stimulate a series of biological events, allowing theranostic and other biomedical applications. So far, there remains a lack of knowledge about the mechanisms governing the nanoparticle-cell membrane interface, especially the impact of ligand exchange, in which molecules on the nanosurface become replaced with components of the cell membrane, resulting in unique interfacial phenomena. Herein, we describe a family of gold nanoparticles (AuNPs) of the same core size (∼13 nm core), modified with 12 different kinds of surface ligands, and the effects of their exchangeable ligands on both nanoparticle-supported lipid bilayers (SLBs) and nanoparticle-natural cell membrane interfaces. The ligands are categorized according to their molecular weight, charge, and bonding modes (physisorption or chemisorption). Importantly, we found that, depending on the adsorption affinity and size of ligand molecules, physisorbed ligands on the surface of NPs can be exchanged with lipid molecules. At a ligand exchange-dominated interface, the AuNPs typically aggregated into an ordered monolayer in the lipid bilayers, subsequently affecting cell membrane integrity, NP uptake efficiency, and the NP endocytosis pathways. These findings advance our understanding of the underlying mechanisms of the biological effects of nanoparticles from a new point of view and will aid in the design of novel, safe, and effective nanomaterials for biomedicine.