Cellular inflammatory infiltrate in pneumonitis induced by a single moderate dose of thoracic x radiation in rats.
Cellular inflammatory infiltrate in pneumonitis induced by a single moderate dose of thoracic x radiation in rats.
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DOI：
10.1667/rr1753.1
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发表时间：
2010-04
影响因子：
3.4
通讯作者：
Medhora M
中科院分区：
文献类型：
--
作者：
Szabo S;Ghosh SN;Fish BL;Bodiga S;Tomic R;Kumar G;Morrow NV;Moulder JE;Jacobs ER;Medhora M
这些研究的目的是描述中等剂量辐射引起的肺炎期间浸润的炎性细胞的特征。两组雄性大鼠（WAG/RijCmcr，8周龄）接受单次10-或15-戈伊剂量的胸部X射线照射;第三组年龄匹配的动物作为对照。只有25%的大鼠在15-戈伊剂量下存活。8周后，对支气管肺泡灌洗液和全肺标本进行细胞学和组织学评价，以确定巨噬细胞、中性粒细胞、淋巴细胞和肥大细胞的分布。接受15戈伊照射的大鼠肺中气道和气道相关中性粒细胞略有增加。肥大细胞（类胰蛋白酶免疫组化检测）增加70%以上，10戈伊和13倍以上，15戈伊后，相当大的类胰蛋白酶泄漏到血管和气道。循环水平的8个炎性细胞因子没有改变后，10戈伊，但似乎减少后，15戈伊。总之，在非致死剂量10戈伊后，肺炎期间细胞炎性浸润仅适度增加，但在15戈伊后肥大细胞浸润显著增加，表明肥大细胞产物的循环水平可能是严重肺炎的有用标志物。
The goal of these studies was to characterize the infiltrating inflammatory cells during pneumonitis caused by moderate doses of radiation. Two groups of male rats (WAG/RijCmcr, 8 weeks old) were treated with single 10- or 15-Gy doses of thoracic X radiation; a third group of age-matched animals served as controls. Only 25% rats survived the 15-Gy dose. Bronchoalveolar lavage fluid and whole lung mounts were subjected to cytological and histological evaluation after 8 weeks for distribution of resident macrophages, neutrophils, lymphocytes and mast cells. There was a modest increase in airway and airspace-associated neutrophils in lungs from rats receiving 15 Gy. Mast cells (detected by immunohistochemistry for tryptase) increased over 70% with 10 Gy and over 13-fold after 15 Gy, with considerable leakage of tryptase into blood vessels and airways. Circulating levels of eight inflammatory cytokines were not altered after 10 Gy but appeared to decrease after 15 Gy. In summary, there were only modest increases in cellular inflammatory infiltrate during pneumonitis after a non-lethal dose of 10 Gy, but there was a dramatic rise in mast cell infiltration after 15 Gy, suggesting that circulating levels of mast cell products may be useful markers of severe pneumonitis.