A HUMAN PARVOVIRUS-LIKE VIRUS INHIBITS HEMATOPOIETIC COLONY FORMATION INVITRO
A HUMAN PARVOVIRUS-LIKE VIRUS INHIBITS HEMATOPOIETIC COLONY FORMATION INVITRO
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DOI：
10.1038/302426a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
YOUNG, NS
中科院分区：
文献类型：
--
作者：
MORTIMER, PP;HUMPHRIES, RK;YOUNG, NS
病毒已被证明可导致动物骨髓发育不全1，2，并与人类骨髓衰竭有关3 -6;然而，直到最近，人类病毒感染和骨髓衰竭之间的特定联系尚未得到证实。1975年，Cossart及其同事在人类血清中发现了一种血清细小病毒样病毒（SPLV，有时称为B19）。该病毒的抗体存在于30-45%的健康成人的血清中（Y. E. Cossart，P. P. Mortimer，未发表的观察）。然而，直接联系的证据来自Pattisonet等人的工作，他发现5名儿童患有镰状细胞病的短暂性再生障碍性危象，并有SPLV 8活动性感染的证据。这种关联后来在牙买加的一系列镰状细胞病和再生障碍性危象儿童中得到证实9。我们研究了含病毒物质对造血的影响，用体外集落形成试验寻找SPLV在骨髓再生障碍中作用的直接证据。我们在这里表明，含SPLV的血清抑制培养中的红细胞生成。此外，在一名患有遗传性球形红细胞增多症的儿童中，患者血清对红细胞生成的强烈抑制作用与病毒的存在有关。
Viruses have been shown to cause bone marrow aplasia in animals1,2and have been implicated in bone marrow failure in man3–6; however, until recently, a specific link between human viral infection and bone marrow failure has not been proven. In 1975 Cossart and colleagues found a serum parvovirus-like virus (SPLV, sometimes referred to as B19) in human serum7. Antibody to this virus is present in the sera of 30–45% of healthy adults (Y. E. Cossart, P. P. Mortimer, unpublished observations). However, evidence for a direct link came from work by Pattisonet al.who found five children with transient aplastic crisis of sickle cell disease and evidence of active infection with SPLV8. This association was later confirmed in a large series of children with sickle cell disease and aplastic crisis in Jamaica9. We have studied the effects of virus-containing material on haematopoiesis, usingin vitrocolony-forming assays to look for direct evidence for a role of SPLV in bone marrow aplasia. We show here that SPLV-containing sera inhibit erythropoiesis in culture. Moreover, in a child with hereditary spherocytosis who developed transient aplastic crisis, a strong inhibitory effect of the patient's serum on erythropoiesis correlated with the presence of virus.