Effect of normolipemic and hyperlipemic serum on biosynthetic response to cyclic stretching of aortic smooth muscle cells.
Effect of normolipemic and hyperlipemic serum on biosynthetic response to cyclic stretching of aortic smooth muscle cells.
复制标题
正常血脂和高血脂血清对主动脉平滑肌细胞循环拉伸生物合成反应的影响。
DOI：
10.1161/01.atv.9.4.446
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Mathews,MB
中科院分区：
文献类型：
--
作者：
Grande,JP;Glagov,S;Bates,SR;Horwitz,AL;Mathews,MB
动脉平滑肌细胞对机械刺激的反应是合成基质大分子。暴露于血清脂质也刺激结缔组织纤维积累。为了评估血清脂质对拉伸应力的生物合成反应的影响，我们将兔主动脉平滑肌细胞培养在纯化的弹性蛋白膜上，在无血清培养基（SFM），正常血脂血清（NLS）或高血脂血清（HLS）的存在下，每分钟循环拉伸和松弛50次。将14 C-脯氨酸掺入脯氨酸和羟脯氨酸中作为蛋白质和胶原蛋白合成的量度。当细胞在塑料培养皿中生长时，与SFM中的合成相比，暴露于NLS或HLS使蛋白质和胶原蛋白的产生增加到相同的程度（NLS为1.7倍，HLS为1.6倍;与SFM相比，p小于0.001）。对于生长在固定弹性蛋白膜上的细胞，与SFM相比，NLS和HLS也增加了蛋白质和胶原蛋白的合成。NLS对蛋白质的作用是HLS的1.35倍，对胶原蛋白的作用是HLS的1.43倍（p <0.03）。与静止对照相比，SFM中的循环拉伸使蛋白质（p小于0.002）和胶原蛋白（p小于0.002）的合成增加一倍，但对NLS中的合成没有影响。然而，在HLS中，环状拉伸将合成提高到与在NLS中发现的相同水平（p小于0.003）。我们的结论是，相对抑制合成的固定膜HLS是不是由于毒性作用，因为HLS增加合成在培养皿和弹性蛋白膜，和放大效应的循环拉伸HLS中看到的SFM是相似的。（250字处删节）
Arterial smooth muscle cells synthesize matrix macromolecules in response to mechanical stimulation. Exposure to serum lipids also stimulates connective tissue fiber accumulation. To assess the effect of serum lipids on the biosynthetic response to tensile stress, we subjected rabbit aortic smooth muscle cells that were cultured on purified elastin membranes to cyclic stretching and relaxation 50 times per minute in the presence of serum-free medium (SFM), normolipemic serum (NLS), or hyperlipemic serum (HLS). Incorporation of 14C-proline into proline and into hydroxyproline was taken as a measure of protein and collagen synthesis. When cells were grown in plastic Petri dishes, exposure to NLS or HLS increased both protein and collagen production to the same extent compared to synthesis in SFM (1.7 times for NLS and 1.6 times for HLS; p less than 0.001 compared to SFM). For cells grown on stationary elastin membranes, NLS and HLS also increased protein and collagen synthesis compared to SFM. The effect of NLS was 1.35 times that of HLS for protein and 1.43 times greater for collagen (p less than 0.03). Cyclic stretching in SFM doubled synthesis for both protein (p less than 0.002) and collagen (p less than 0.002) compared to stationary controls, but had no effect on synthesis in NLS. In HLS, however, cyclic stretching elevated synthesis to the same level as was found in NLS (p less than 0.003). We conclude that the relative inhibition of synthesis on stationary membranes by HLS was not due to a toxic effect, since HLS increased synthesis both in Petri dishes and on elastin membranes, and the amplifying effect of cyclic stretching in HLS was similar to that seen in SFM.(ABSTRACT TRUNCATED AT 250 WORDS)