The Role of Lumbar Sympathetic Nerves in Regulation of Blood Flow to Skeletal Muscle during Anaphylactic Hypotension in Anesthetized Rats.
The Role of Lumbar Sympathetic Nerves in Regulation of Blood Flow to Skeletal Muscle during Anaphylactic Hypotension in Anesthetized Rats.
复制标题
DOI：
10.1371/journal.pone.0150882
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Kurata Y
中科院分区：
文献类型：
--
作者：
Song J;Tanida M;Shibamoto T;Zhang T;Wang M;Kuda Y;Kurata Y
在低血容量性休克期间，骨骼肌血流可通过血管收缩重新分配至重要器官，血管收缩部分由支配交感神经活动的激活引起。然而，这一机制是否在过敏性休克中起作用还不清楚。本文测定了麻醉大鼠过敏性低血压时股动脉血流量（FBF）和腰交感神经活动（LSNA）的变化。将麻醉的Sprague-Dawley大鼠随机分配到以下组（n = 7/组）：（1）非致敏组，（2）过敏组，（3）过敏-腰交感神经切除术（LS）组和（4）过敏-窦主动脉去神经（SAD）组。致敏大鼠静脉注射卵清蛋白抗原诱导过敏反应。连续监测全身动脉压（SAP）、心率（HR）、中心静脉压（CVP）、FBF和LSNA。过敏反应组注射抗原后LSNA、HR升高，SAP、FBF降低。在过敏-SAD组中，LSNA在早期没有显著变化，但SAP和FBF的反应与过敏组相似。在过敏-LS组中，在过敏性低血压期间，FBF和SAP均与过敏反应组相似地降低。这些结果表明，LSNA通过压力感受器反射增加，但这种交感兴奋或LS不影响抗原诱导的FBF或SAP减少。腰交感神经不参与麻醉大鼠过敏性低血压时后肢血流或全身血压的调节。
During hypovolemic shock, skeletal muscle blood flow could be redistributed to vital organs via vasoconstriction in part evoked by activation of the innervating sympathetic nerve activity. However, it is not well known whether this mechanism operates during anaphylactic shock. We determined the femoral artery blood flow (FBF) and lumbar sympathetic nerve activity (LSNA) mainly regulating the hindquater muscle blood flow during anaphylactic hypotension in anesthetized rats. Anesthetized Sprague-Dawley rats were randomly allocated to the following groups (n = 7/group): (1) non-sensitized, (2) anaphylaxis, (3) anaphylaxis-lumbar sympathectomy (LS) and (4) anaphylaxis-sinoaortic denervation (SAD) groups. Anaphylaxis was induced by an intravenous injection of the ovalbumin antigen to the sensitized rats. The systemic arterial pressure (SAP), heart rate (HR), central venous pressure (CVP), FBF and LSNA were continuously measured. In the anaphylaxis group, LSNA and HR increased, while SAP and FBF decreased after antigen injection. In the anaphylaxis-SAD group, LSNA did not significantly change during the early phase, but the responses of SAP and FBF were similar to those in the anaphylaxis group. In the anaphylaxis-LS group, both FBF and SAP decreased similarly to the anaphylaxis group during anaphylactic hypotension. These results indicated that LSNA increased via baroreceptor reflex, but this sympathoexcitation or LS did not affect antigen-induced decreases in FBF or SAP. Lumbar sympathetic nerves are not involved in regulation of the blood flow to the hindlimb or systemic blood pressure during anaphylactic hypotension in anesthetized rats.