Mycobacterium tuberculosis gene expression during adaptation to stationary phase and low-oxygen dormancy
Mycobacterium tuberculosis gene expression during adaptation to stationary phase and low-oxygen dormancy
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DOI：
10.1016/j.tube.2004.02.003
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发表时间：
2004-01-01
期刊：
影响因子：
3.2
通讯作者：
Schoolnik, GK
中科院分区：
文献类型：
--
作者：
Voskuil, MI;Visconti, KC;Schoolnik, GK
结核分枝杆菌在非增殖期间持续存在的固有机制是理解潜伏疾病期间杆菌生理学的核心。我们已经使用全基因组表达谱来揭示由M.结核分枝杆菌的两种常见模式。结核病不扩散。这些模型中的第一个是标准生长曲线，其中基因表达变化从指数生长到稳定期的过渡。在第二个模型中，我们遵循M.在从有氧生长到厌氧非复制持续状态的过渡期间，结核病。从这些实验中最引人注目的发现是整个DosR“休眠”调节子在向厌氧状态的长过渡期间在大约20天内的强烈诱导。这是由静音的整体响应充气静止期只有一个部分休眠调节子响应形成对比。从这里的结果，我们得出结论，呼吸限制环境的缺氧NRP模型重建至少一个基本因素，为M。结核病编码了一个决定性的适应程序。(C)2004爱思唯尔有限公司保留所有权利。
The innate mechanisms used by Mycobacterium tuberculosis to persist during periods of non-proliferation are central to understanding the physiology of the bacilli during latent disease. We have used whole genome expression profiling to expose adaptive mechanisms initiated by M. tuberculosis in two common models of M. tuberculosis non-proliferation. The first of these models was a standard growth curve in which gene expression changes were followed from exponential growth through the transition to stationary phase. In the second model, we followed the adaptive process of M. tuberculosis during transition from aerobic growth to a state of anaerobic non-replicating persistence. The most striking finding from these experiments was the strong induction of the entire DosR "dormancy" regulon over approximately 20 days during the long transition to an anaerobic state. This is contrasted by the muted overall response to aerated stationary phase with only a partial dormancy regulon response. From the results presented here we conclude that the respiration-limited environment of the oxygen-depleted NRP model recreates at least one fundamental factor for which the genome of M. tuberculosis encodes a decisive adaptive program. (C) 2004 Elsevier Ltd. All rights reserved.