In vivo biocompatibility studies. V. In vivo leukocyte interactions with Biomer.
In vivo biocompatibility studies. V. In vivo leukocyte interactions with Biomer.
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体内生物相容性研究。
DOI：
10.1002/jbm.820180917
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Anderson,JM
中科院分区：
文献类型：
--
作者：
Marchant,RE;Miller,KM;Anderson,JM
利用笼式植入系统定量和定性地表征内白细胞与铸型生物聚合物的相互作用。扫描电子显微镜（SEM）结合细胞化学染色程序用于研究在21天的植入期间白细胞/生物聚合物界面以及炎症渗出液中的细胞事件。扫描电镜（SEM）用于表征生物聚合物表面的白细胞形态，细胞化学染色用于区分白细胞计数，并显示细胞内碱性和酸性磷酸酶活性。结果表明，随着植入时间的延长，生物聚合物表面的白细胞密度逐渐降低。多核异物体巨细胞的种群密度随时间保持不变，但每个巨细胞的细胞核数增加。差异分析显示，与渗出液中的其他白细胞相比，巨噬细胞优先粘附在生物聚合物表面。所有粘附的白细胞（包括巨细胞）的吞噬能力随着时间的推移而下降，这与扫描电镜观察到的白细胞形态变化相对应。
A cage implant system was utilized to quantitatively and qualitatively characterizein vivoleukocyte interactions with cast Biomer. Scanning electron microscopy (SEM) in conjunction with cytochemical staining procedures were used to investigate the cellular events at the leukocyte/Biomer interface as well as in the inflammatory exudate over a 21‐day implantation period. SEM was used to characterize leukocyte morphology on the Biomer surface and the cytochemical stains were used to differentially count leukocytes and to demonstrate intracellular alkaline and acid phosphatase activity. The results showed that the population density of leukocytes on the Biomer surface diminished with implantation time. The population density of multinucleated foreign body giant cells remained constant with time, while the numbers of nuclei per giant cell increased. The differential analysis revealed that macrophages preferentially adhered to the Biomer surface compared to other leukocytes in the exudate. The phagocytic capability of all adherent leukocytes, including giant cells, decreased with time and this corresponded to changes in leukocyte morphology observed with SEM.