Changes in peroxidase activity and isoenzymes in spruce needles after exposure to different concentrations of cadmium
Changes in peroxidase activity and isoenzymes in spruce needles after exposure to different concentrations of cadmium
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DOI：
10.1016/s0098-8472(00)00059-9
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发表时间：
2000-10-01
影响因子：
5.7
通讯作者：
Mutavdzic, D
中科院分区：
文献类型：
--
作者：
Radotic, K;Ducic, T;Mutavdzic, D
研究了在土壤中添加1~21 mg kg(-1)镉对2年生云杉针叶愈创木酚过氧化物酶活性、同工酶和金属含量的影响。在镉暴露后，观察到可溶部分的活性先增加后降低。它们的同工酶图谱也发生了平行的变化。在长时间的金属处理下，细胞壁结合的过氧化物酶活性明显增加。结果表明，过氧化物酶活性和同工酶谱图可以用来评价云杉幼苗防御系统的一部分抵御金属胁迫的能力。(C)2000 Elsevier Science B.V.保留所有权利。
We studied the guaiacol peroxidase activity, isoenzyme pattern and metal content in the needles of 2-year-old spruce grown on soils supplemented with cadmium concentrations from 1 to 21 mg kg(-1). Following exposure to cadmium, an initial increase and subsequent decrease in the activity of the soluble fraction was observed. A parallel change of their isoenzyme pattern occurred. An increase of the cell wall-bound peroxidase activity under prolonged metal treatment was evident. The results obtained show that peroxidase activity and isoenzyme pattern could be used to evaluate the capacity of one part of the defense system in spruce seedlings to withstand metal stress. (C) 2000 Elsevier Science B.V. All rights reserved.