The occurrence and immunology of peptidylarginine deiminase and its preparation from bovine epidermis by an improved method.
The occurrence and immunology of peptidylarginine deiminase and its preparation from bovine epidermis by an improved method.
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肽基精氨酸脱亚胺酶及其改良方法制备牛表皮的发生和免疫学。
DOI：
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发表时间：
1985
影响因子：
6.5
通讯作者：
H. Baden
中科院分区：
文献类型：
--
作者：
J. Kubilus;H. Baden
在每个脊椎动物类别的至少一个代表的某些组织中发现了肽基精氨酸脱亚胺酶活性，这表明该酶在脊椎动物中普遍存在。采用精氨酸琼脂糖亲和层析三步法，大大改进了牛表皮肽基精氨酸脱亚胺酶的纯化方法。纯化的酶制剂经十二烷基硫酸钠电泳法检测，含有一条70-75kD的条带和几个次要成分。用间接免疫荧光法检测牛脑酶多克隆抗体与人和新生大鼠皮肤的交叉反应。这种交叉反应通过抗体对纯化的脑酶的吸收而显著减弱。
Peptidylarginine deiminase activity has been found in some tissues from at least one representative of each vertebrate class, suggesting that the occurrence of the enzyme throughout the vertebrates is widespread. Using a three-step procedure including affinity chromatography on arginine agarose, a greatly improved purification of bovine epidermal peptidylarginine deiminase is presented. The purified enzyme preparation contains a 70-75 kD band and several minor components when examined by sodium dodecyl sulfate electrophoresis. A polyclonal antibody raised to the enzyme of bovine brain cross-reacts with human and newborn rat epidermis by indirect immunofluorescence. This cross-reactivity is markedly diminished by absorption of the antibody to the purified brain enzyme.