Microbial Signatures Associated with Oropharyngeal and Oral Squamous Cell Carcinomas.
Microbial Signatures Associated with Oropharyngeal and Oral Squamous Cell Carcinomas.
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DOI：
10.1038/s41598-017-03466-6
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发表时间：
2017-06-22
影响因子：
4.6
通讯作者：
Robertson ES
中科院分区：
文献类型：
--
作者：
Banerjee S;Tian T;Wei Z;Peck KN;Shih N;Chalian AA;O'Malley BW;Weinstein GS;Feldman MD;Alwine J;Robertson ES
微生物组基本上是人体中最独特的器官之一。生态失调可导致严重的炎症反应，并导致导致肿瘤事件的发病机制。我们使用泛病原体阵列技术（PathoChip）结合下一代测序，以建立人类口腔和口咽鳞状细胞癌（OCSCC/OPSCC）特有的微生物特征。检测到DNA和RNA病毒的特征，包括致癌病毒、革兰氏阳性和阴性细菌、真菌和寄生虫。聚类和拓扑分析确定了与OCSCC/OPSCC相关的2组不同的微生物特征。结果通过探针捕获下一代测序进行验证;其中的数据还提供了微生物基因组插入的整合位点和染色体热点的综合图谱。这些微生物特征及其整合位点的鉴定可能为OCSCC/OPSCC的诊断和预后提供生物标志物，以及研究其在OCSCC/OPSCC中的潜在作用的新途径。
The microbiome is fundamentally one of the most unique organs in the human body. Dysbiosis can result in critical inflammatory responses and result in pathogenesis contributing to neoplastic events. We used a pan-pathogen array technology (PathoChip) coupled with next-generation sequencing to establish microbial signatures unique to human oral and oropharyngeal squamous cell carcinomas (OCSCC/OPSCC). Signatures for DNA and RNA viruses including oncogenic viruses, gram positive and negative bacteria, fungi and parasites were detected. Cluster and topological analyses identified 2 distinct groups of microbial signatures related to OCSCCs/OPSCCs. Results were validated by probe capture next generation sequencing; the data from which also provided a comprehensive map of integration sites and chromosomal hotspots for micro-organism genomic insertions. Identification of these microbial signatures and their integration sites may provide biomarkers for OCSCC/OPSCC diagnosis and prognosis as well as novel avenues for study of their potential role in OCSCCs/OPSCCs.