Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching
Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching
复制标题
快速爬行的细胞类型会迁移以避免周期性基质拉伸的方向
DOI：
10.1080/19336918.2015.1129482
复制
发表时间：
2016
影响因子：
3.2
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Okimura;C.;Ueda;K.;Sakumura;Y. and Iwadate;Y.
为了研究基质的机械刺激与相关细胞功能之间的关系，最有用的技术之一是通过周期性拉伸弹性基质来施加机械刺激。响应于这种刺激，盘基网柄藻细胞在垂直于拉伸方向的方向上迁移。然而，定向迁移的起源、在垂直于拉伸方向的方向上更高的迁移速度或迁移方向切换到垂直于拉伸方向的更高概率仍然未知。在这项研究中，我们施加周期性的拉伸刺激，以嗜酸性粒细胞样分化的HL-60细胞，迁移垂直于拉伸的方向。对HL-60细胞和Dictyostelium细胞的运动轨迹进行了详细的分析，发现细胞向垂直于拉伸方向迁移的可能性较大，这是导致细胞定向迁移的主要原因。这种定向迁移似乎是快速爬行细胞采用的一种策略，在这种策略中，它们不会在它们想要去的方向上迁移得更快，而是迁移以避免它们不想去的方向。
To investigate the relationship between mechanical stimuli from substrata and related cell functions, one of the most useful techniques is the application of mechanical stimuli via periodic stretching of elastic substrata. In response to this stimulus,Dictyostelium discoideumcells migrate in a direction perpendicular to the stretching direction. The origins of directional migration, higher migration velocity in the direction perpendicular to the stretching direction or the higher probability of a switch of migration direction to perpendicular to the stretching direction, however, remain unknown. In this study, we applied periodic stretching stimuli to neutrophil-like differentiated HL-60 cells, which migrate perpendicular to the direction of stretch. Detailed analysis of the trajectories of HL-60 cells andDictyosteliumcells obtained in a previous study revealed that the higher probability of a switch of migration direction to that perpendicular to the direction of stretching was the main cause of such directional migration. This directional migration appears to be a strategy adopted by fast-crawling cells in which they do not migrate faster in the direction they want to go, but migrate to avoid a direction they do not want to go.