Characterization of the Hog1 MAPK pathway in the entomopathogenic fungus Beauveria bassiana
Characterization of the Hog1 MAPK pathway in the entomopathogenic fungus Beauveria bassiana
复制标题
昆虫病原真菌白僵菌 Hog1 MAPK 通路的表征
DOI：
10.1111/1462-2920.13671
复制
发表时间：
2017-05-01
影响因子：
5.1
通讯作者：
Feng, Ming-Guang
中科院分区：
文献类型：
--
作者：
Liu, Jing;Wang, Zhi-Kang;Feng, Ming-Guang
酵母渗透调节所需的高渗透性甘油（HOG）途径依赖于有丝分裂原激活的蛋白激酶（MAPK）HOG1 HOG1 CASCADE，其中包括MAPKKKS SSK2/SSK22和STE11和STES11在MAPKK PBS上收敛。在这里，我们展示了一个HOG1级联，其中包括Beauveria Bassiana的独特Mapkkk SSK2。 SSK2，PBS2和HOG1突变体出现了对高渗透压和对氟二尼尔杀菌剂的高敏性，而两个标志性表型在Ste11中反转。在所有缺失突变体中抗氧化剂表型都不同，但在三个突变体中，对热休克的敏感性增加和对细胞壁扰动的敏感性也会增加，但在Ste11中也不会发生。有趣的是，Ste11中存在由渗透，氧化和热线索诱导的HOG1磷酸化信号，但在SSK2和PBS2中不存在。此外，在Ste11和SSK2中，具有不同碳/氮来源的最小培养基的营养生长要比在PBS2和HOG1中受到更大的抑制，尽管所有突变体在标准培养基上遭受了相似但严重的分生物缺陷。在其他突变体中毒性差异降低时，在Ste11中废除了正常的宿主感染。我们的发现将Ste11排除在HOG1级联反应之外，该级联可以调节B. Bassiana以及其他丝状真菌的多种压力反应和环境适应。
High-osmolarity glycerol (HOG) pathway required for yeast osmoregulation relies upon the mitogen-activated protein kinase (MAPK) Hog1 cascade that comprise the MAPKKKs Ssk2/Ssk22 and Ste11 converging on the MAPKK Pbs2. Here we show a Hog1 cascade with the unique MAPKKK Ssk2 acting in Beauveria bassiana. Hypersensitivity to high osmolarity and high resistance to fludioxonil fungicide appeared in Δssk2, Δpbs2 and Δhog1 mutants whereas the two hallmark phenotypes were reversed in Δste11. Increased sensitivity to heat shock and decreased sensitivity to cell wall perturbation also occurred in the three mutants but not in Δste11 although antioxidant phenotypes were different in all deletion mutants. Intriguingly, signals of Hog1 phosphorylation induced by osmotic, oxidative and thermal cues were present in Δste11 but absent in Δssk2 and Δpbs2. Moreover, vegetative growth on minimal media with different carbon/nitrogen sources was much more suppressed in Δste11 and Δssk2 than in Δpbs2 and Δhog1 although all mutants suffered similar, but severe, conidiation defects on a standard medium. Normal host infection was abolished in Δste11 while virulence was differentially attenuated in other mutants. Our findings exclude Ste11 from the Hog1 cascade that regulates multiple stress responses and environmental adaptation of B. bassiana and perhaps other filamentous fungi.