Rabbit umbilical cord mesenchymal stem cells: A new option for tissue engineering
Rabbit umbilical cord mesenchymal stem cells: A new option for tissue engineering
复制标题
兔脐带间充质干细胞：组织工程的新选择
DOI：
10.1002/jgm.3282
复制
发表时间：
2020-11-15
影响因子：
3.5
通讯作者：
Liu, Lei
中科院分区：
文献类型：
--
作者：
Li, Kai-De;Wang, Yi;Liu, Lei
背景：在大规模研究或临床试验中，组织工程细胞的来源和可用性需要特别关注。我们提出了应用兔脐带间充质干细胞用于此purpose.Methods的想法：在这里，兔脐带的结构进行了分析，并与人脐带，宏观和组织学。其次，采用混合淋巴细胞反应、流式细胞术和酶联免疫吸附试验对兔脐带间充质干细胞进行体外分离、培养和鉴定，并对其增殖活性和免疫学特性进行研究。此外，我们评估了双相磷酸钙陶瓷支架与兔脐带间充质干细胞接种在大鼠颅骨缺损models.Results的影响，使用多种技术，包括放射学，组织学和免疫组化：在体外研究证明了高水平的增殖和多向分化潜能的兔脐带间充质干细胞。兔脐带间充质干细胞对同种异体和异种免疫反应均表现出低免疫原性和免疫抑制能力。体内实验结果表明，兔脐带间充质干细胞具有促进异种骨成骨的作用。结论：兔脐带间充质干细胞有可能成为组织工程的新来源。
Background: The source and availability of cells for tissue engineering in large scale research or clinical trials requires special attention. We propose the idea of applying rabbit umbilical cord mesenchymal stem cells for this purpose.Methods: Here, the structure of the rabbit umbilical cord was analyzed and compared to that of human umbilical cord, both macroscopically and histologically. Next, we isolated, cultured and identified the proliferative activity and immunological characteristics of rabbit umbilical cord mesenchymal stem cells in vitro using mixed lymphocyte reaction, flow cytometry and an enzyme-linked immunosorbent assay. Furthermore, we evaluated the effects of biphasic calcium phosphate ceramic scaffolds seeded with rabbit umbilical cord mesenchymal stem cells in rat cranial defect models using multiple techniques, including radiological, histological and immunohistochemistry.Results: In vitro studies demonstated a high level of proliferation and multi-lineage differentiation potential in rabbit umbilical cord mesenchymal stem cells. Rabbit umbilical cord mesenchymal stem cells exibited low immunogenicity properties and immune suppression capability with respect to both the allogeneic and xenogeneic immune response. The results of the in vivo study showed that rabbit umbilical cord mesenchymal stem cells could promote osteogenesis in heterogeneous hosts.Conclusions: The rabbit umbilical cord mesenchymal stem cells may be a new source for tissue engineering.