The Protective Role of Telmisartan Against Nephrotoxicity Induced by X-ray Contrast Media in Rat Model
The Protective Role of Telmisartan Against Nephrotoxicity Induced by X-ray Contrast Media in Rat Model
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DOI：
10.1080/02841850902995544
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发表时间：
2009-01-01
期刊：
影响因子：
1.3
通讯作者：
Peng, You Ming
中科院分区：
文献类型：
--
作者：
Duan, Shao Bin;Wang, Yu Hui;Peng, You Ming
背景资料：造影剂肾病是诊断和介入治疗的严重并发症，目的：评价高渗和低渗造影剂（HOCM，LOCM）对SD大鼠肾脏的毒性作用。材料与方法：将60只雄性大鼠随机分为6组，每组10只。除对照组外，所有大鼠均给予甘油以诱导肾损伤。HOCM（泛影葡胺）或LOCM（碘海醇）（10 ml/kg体重，300 mg I/ml）通过尾静脉给药。采用全自动生化分析仪测定血清肌酐水平。采用荧光法和放射免疫法分别检测肾组织Caspase-3活性和血管紧张素II（Angiotensin II，Ang II）水平。用苏木精-伊红染色和脱氧尿苷缺口末端标记法检测肾损伤程度。结果：注射泛影葡胺后，大鼠血清肌酐水平升高（P
Background: Contrast-induced nephropathy is a serious complication of diagnostic and interventional procedures.Purpose: To evaluate the nephrotoxicity of high-and low-osmolar contrast media (HOCM, LOCM) on kidneys in Sprague-Dawley rats. Telmisartan was administered to confirm its protective role against nephrotoxicity induced by contrast media.Material and Methods: Sixty male rats were randomly divided into six groups (n = 10/group). Glycerin was given to all rats except controls to induce renal injury. HOCM (diatrizoate) or LOCM (iohexol) (10 ml/kg b.w., 300 mg I/ml) was given through a caudal vein. Serum creatinine level was measured by an automatical biochemical analyzer. Caspase-3 activity and Angiotensin II (Ang II) level of renal tissue were detected by fluorometric method and radioimmunoassay, respectively. The renal injury was also assessed by hematoxylin and eosin and TdT-mediated deoxyuridine nick end-labeling staining.Results: In diatrizoate-injected rats, serum creatinine level was increased (P